XÁC ĐỊNH SẢN PHẨM ĐÔNG TRÙNG HẠ THẢO DỰA VÀO CHỈ THỊ ITS1 CỦA NẤM CODYCEPTS SINENSIS VÀ COI CỦA ẤU TRÙNG BƯỚM THUỘC HỌ HEPIALIDAE

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Tác giả: Quang Trung Lê, Chi Mai Nguyễn, Tường Vân Nguyễn, Mỹ Linh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.393-400

Bộ sưu tập: Metadata

ID: 339701

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Đông trùng hạ thảo là sản phẩm do nấm Cordyceps sinensis ký sinh và phát triển trong ấu trùng thuộc họ Hepialidae từ mùa đông đến mùa hè trên các vùng núi cao >
3000m so với mặt biển ở Trung Quốc, Nepal… Đông trùng hạ thảo có vai trò kinh tế và y học cao nên thường bị pha trộn với nhộng trùng thảo Codycepts militaris ký sinh trên ấu trùng của nhiều loài bướm khác nhau và có thể sản xuất quanh năm trong môi trường tự nhiên hoặc nhân tạo. Trong nghiên cứu này, 10 mẫu đông trùng hạ thảo được xác định dựa vào chỉ thị ITS1 của nấm Codycepts và COI của ấu trùng bướm Hepialidae, tằm ăn lá dâu Bombyx mori và tằm ăn lá sắn Samia ricini. Kết quả phân tích bằng cây chủng loại và mức tương đồng về trình tự DNA của các chỉ thị cho thấy 10 mẫu đều bị trộn lẫn giữa đông trùng hạ thảo và nhộng trùng thảo, trong đó 5 mẫu chứa cả nấm Metacodycepts neoginnii. Toàn bộ 10 mẫu đều chứa đông trùng hạ thảo của ấu trùng Hepialidae, nhưng có 5 mẫu còn chứa cả ấu trùng B. mori. Như vậy, cả 10 mẫu đều có thành phần không thuần nhất. Kết quả thu được đã cung cấp phương pháp xác định được thành phần trong một số sản phẩm đông trùng hạ thảo đã qua chế biến sử dụng các chỉ thị phân tử.Products of caterpillar fungus are the complex of Cordyceps sinensis species parasitic to larvae of species of Hepialidae family and developing from winter to summer in high mountains of China, Nepal… Caterpillar fungus has high economic benefits because of its medicinal properties. Therefore, it could be mixed with clavicipitaceous fungi, which are parasitic on larvae of different moth hosts and are produced throughout the year in natural condition or artificial media. In this research, 10 caterpillar fungus samples in capsules were examined based on markers of ITS1 of Codycepts species and COI of Hepialidae and Bombyx mori larvae. Phylogenetic and homology level analyses based on nucleotide polymorphism of the markers revealed that 10 samples contained both caterpillar fungus of C. sinensis and clavicipitaceous fungi of Codycepts militaris and Metacodycepts neoginnii. Similarly, powder of Hepialidae larvae was found in all 10 samples, but five of which were also containing that of B. mori. Therefore, 10 samples were ditermined as no pure caterpillar fungus from C. sinensis and five of them were wrong about Hepialidae hosts. The results in this study suggest the effective uses of molecular markers in identifying possible sources of processed caterpillar fungus products.

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