ĐÁNH GIÁ KHẢ NĂNG ỨC CHẾ NẤM FUSARIUM OXYSPORUM CỦA 12 LOẠI NANO TINH DẦU THỰC VẬT KHÁC NHAU VÀ CÁC CÔNG THỨC KẾT HỢP CỦA CHÚNG

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Tác giả: Minh Hiệp Nguyễn, Thị Ngọc Mai Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.430-436

Bộ sưu tập: Báo, Tạp chí

ID: 339709

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu nhằm mục đích đánh giá hiệu quả ức chế nấm Fusarium oxysporum trong điều kiện in vitro của 12 loại tinh dầu thực vật ở dạng nano (EO-LNs) và hệ kết hợp hai và ba của 4 loại tinh dầu tốt nhất. Đầu tiên, 12 EO-LNs đã được chuẩn bị bằng phương pháp đồng hóa tốc độ cao kết hợp với giảm kích thước bằng sóng siêu âm. Hệ tinh dầu kết hợp hai và kết hợp ba được tạo thành khi trộn các EO-LNs với tỉ lệ bằng nhau. Hiệu quả kháng nấm in vitro của 12 EO-LNs và dạng kết hợp của chúng được đánh giá bằng phương pháp đĩa thạch. Kết quả chỉ ra rằng, 12 EO-LNs đã được tổng hợp thành công với kích thước hạt trung bình ở mức nano và thế zeta lớn hơn -42 mV. Kết quả cũng cho thấy, dạng kết hợp của EO-LNs cho hiệu quả ức chế nấm F. oxysporum tốt hơn so với dạng đơn lẻ tương ứng. Đặc biệt, naTS cho thấy hiệu quả kháng nấm tốt nhất trong điều kiện in vitro với hiệu quả ức chế nấm đạt 88,14% ở độ pha loãng 500 lần. Do đó, naTS có tiềm năng ứng dụng như là thuốc trị nấm sinh học chống lại nấm F. oxysporum.The study aims to evaluate the in vitro antifungal activity against Fusarium oxysporum of 12 plant essential oils encapsulated nanoemulsion (EO-LNs) and their binary and ternary combined formulations of the 4 EOs having the highest in vitro antifungal activity. Firstly, 12 EO-LNs were prepared by a combining method of homogenization and sonication. Their binary and ternary combined formulations were also prepared by mixing with an equal volume ratio of each EO-LNs. In vitro antifungal activity of 12 EO-LNs and their combined formulations was evaluated using petri dish assay. The results indicated that 12 EO-LNs and their combined formulations were successfully prepared with the mean particle size at the nanoscale and the zeta potential greater than -42 mV. The results also showed that the combined formulations of EO-LNs had better antifungal activity against F. oxysporum compared to the corresponding individual EO-LNs. Specially, naTS exhibited the highest in vitro antifungal effect with the growth inhibition value of 88.14% at 500 times dilution. Therefore, naTS has a great potential application as an effective biofungicide against F. oxysporum.

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