HOẠT TÍNH ỨC CHẾ TẾ BÀO UNG THƯ GAN HEPG2 CỦA CAO CHIẾT CÂY ELSHOLTZIA CILIATA (THUNB.) HYLAND

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Tác giả: Quốc Khánh Cao, Thị Thanh Hương Lê, Văn Linh Mai, Phú Hùng Nguyễn, Ngô Minh Châu Phạm, Văn Khang Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.442-449

Bộ sưu tập: Metadata

ID: 339711

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Liver cancer is the leading cancer causing death in the world. Studies on the anti-cancer potential of many other herbal species are of current interest. Elsholtzia ciliata (Thunb.) Hyland belongs to the genus Elsholtzia and is commonly used as a spice. It is also used as a remedy for colds and flu. However, its ability to inhibit liver cancer cells has not been studied. In this study, the methods of culture, cytotoxicity analysis, cell cycle and apoptosis are applied to evaluate the anti-cancer ability of Elsholtzia ciliata extract. The results showed that the leaf extract of Elsholtzia ciliata changed cell morphology at concentrations from 100 - 200 µg/mL compared to control. The extract effectively inhibited the proliferation of HepG2, the percentage of viable cells reduced to 41 ± 10.5% (at concentration of 100 µg/mL) and 20.4 ± 7.7% (at concentration of 200 µg/mL) compared to the control (P <
0.05). The extract stopped the cell cycle at G0/G1 phase (62.1 ± 3.5% vs 51.3 ± 4.2%, P <
0.05) and induced cellular apoptosis (P <
0.05). This study shows that Elsholtzia ciliata is a potential herb against liver cancer cells.Ung thư gan là loại ung thư đứng đầu về tỷ lệ gây chết. Các nghiên cứu về tiềm năng chống ung thư từ nhiều loài thảo dược khác nhau đang được quan tâm hiện nay. Elsholtzia ciliata (Thunb.) Hyland thuộc chi Elsholtzia được sử dụng phổ biến như một loại gia vị và vị thuốc chữa cảm cúm. Tuy nhiên, khả năng ức chế tế bào ung thư gan của nó còn chưa được nghiên cứu. Trong nghiên cứu này, các phương pháp nuôi cấy, phân tích độc tính tế bào, chu kỳ tế bào và apoptosis được áp dụng để đánh giá khả năng kháng ung thư của cao chiết từ cây Elsholtzia ciliata. Kết quả cho thấy, cao chiết từ lá của cây Elsholtzia ciliata đã làm thay đổi hình thái tế bào ở nồng độ từ 100 - 200 µg/mL. Cao chiết đã kìm hãm hiệu quả sự tăng sinh của HepG2, tỷ lệ tế bào sống giảm xuống còn 41 ± 10,5% (ở nồng độ 100 µg/mL) và 20,4 ± 7,7% (ở nồng độ 200 µg/mL) so với đối chứng (P <
0,05). Cao chiết đã làm dừng chu kỳ tế bào ở pha G0/G1 (62,1 ± 3,5% so với 51,3 ± 4,2%, P <
0,05) và gây chết tế bào theo con đường apoptosis (P <
0,05). Nghiên cứu này đã chỉ ra rằng, Elsholtzia ciliata là thảo dược tiềm năng chống lại tế bào ung thư gan.

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