PHÂN TÍCH VÙNG GENE LỤC LẠP, rbcL, PHÂN LẬP TỪ CÂY LAN TAI CÁO (Hoya parasitica (Roxb.) Wall. ex Wight)

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Tác giả: Hoàng Mậu Chu, Việt Cường Hoàng, Thị Hoàng Anh Mai, Quang Tân Từ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.474-481

Bộ sưu tập: Metadata

ID: 339720

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Hoya parasitica (Roxb.) Wall. ex Wight), an epiphytic species, is an ornamental and precious medicinal plant. H. parasitica contains many biologically active pharmaceutical substances that are used to treat some human diseases. H. parasitica is morphologically similar to some species of the Hoya genus and the Asclepiadaceae family. How to identify the species and genera in the Asclepiadaceae family when the morphology, growth, and development of the plants are incomplete or the plant specimen is deformed or in powder form was the question posed for this study. In this work, the rbcL gene was isolated and sequenced, analyzed the genetic relationship of H. parasitica was at the species and genera levels by PCR method, nucleotide sequencing, and molecular evolutionary analysis. The analysis showed that the rbcL gene has 547 nucleotides, the coverage is from 99 - 100%, and the similarity rate with the rbcL sequences of other species in the Asclepiadaceae family on GenBank ranges from 98.90 - 99.82%. The phylogenetic tree based on the rbcL sequence of species of the Hoya genus and species of the Asclepiadaceae family has been established. The rbcL gene sequence may be a candidate for chloroplast DNA barcoding for genera-level identification.Lan tai cáo (Hoya parasitica (Roxb.) Wall. ex Wight), một loài thực vật sống phụ sinh, vừa là cây cảnh, vừa là cây dược liệu quý. Lan tai cáo chứa nhiều dược chất có hoạt tính sinh học có tác dụng chữa trị một số bệnh ở người. Lan tai cáo có hình thái tương tự một số loài thuộc chi Hoya và họ Thiên lý. Làm thế nào để nhận diện được các loài, các chi trong họ Thiên lý khi hình thái, sự sinh trưởng và phát triển của cây chưa đầy đủ, hoặc mẫu cây bị biến dạng hay ở dạng bột là câu hỏi đặt ra cho nghiên cứu này. Trong công trình này, gene rbcL được phân lập, giải trình tự và phân tích mối quan hệ di truyền của Lan tai cáo ở cấp độ loài và chi bằng phương pháp PCR, giải trình tự nucleptide và phân tích tiến hoá phân tử. Kết quả phân tích cho thấy, gene rbcL có 547 nucleotide, có độ che phủ từ 99 - 100% và tỷ lệ tương đồng với các trình tự rbcL của các loài khác trong họ Thiên lý trên GenBank từ 98,90 - 99,82%. Cây phát sinh chủng loại dựa trên trình tự rbcL của các loài thuộc chi Hoya và các loài thuộc họ Thiên lý đã được thiết lập. Trình tự gen rbcL có thể là ứng cử viên mã vạch DNA lục lạp để định danh ở cấp độ chi.

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