PHÂN BIỆT MỘT SỐ LOÀI LAN HÀI CÓ QUAN HỆ GẦN GŨI VÀ HÌNH THÁI TƯƠNG ĐỒNG BẰNG CHỈ THỊ DNA BARCODE

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Tác giả: Hoàng Mậu Chu, Tiến Phát Đỗ, Thị Hải Yến Nguyễn, Thị Loan Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.113-121

Bộ sưu tập: Báo, Tạp chí

ID: 339741

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Paphiopedilum is a genus in the subfamily Cypripedioideae with the highest diversity and division, divided into many subgenus and sections. Species in these sections are very closely related to each other, especially having morphological similarities. Currently, several approaches have been applied in determining taxonomy, from morphological-based techniques to classification by molecular markers or a combination of methods, in which DNA barcode presents many advantages. Four species in this study include Paphiopedilum henryanum, Paphiopedilum coccinaum, Paphiopedilum helenae and Paphiopedilum × hermannii belong to the standard section (Paphiopedilum) of the subgenus Paphiopedilum, which are highly similar morphological characteristics. This paper presents the results of plant morphological analysis of these species of venus orchid to analyze two DNA barcode sequences ITS and trnH-psbA to identify them. Our results indicated that morphological characteristics can be used to identify Comedy species in the study. The results of DNA barcode analysis of two ITS and trnH-psbA markers showed that they were suitable for studying the taxonomy of venus orchids, but they were not suitable for identifying each species except for the hybrid species Paphiopedilum × hermannii.Paphiopedilum là một chi có độ đa dạng và phân hóa cao nhất trong phân họ lan Hài, được chia thành nhiều phân chi và section. Các loài trong cùng section có quan hệ rất gần gũi với nhau, đặc biệt có sự tương đồng về hình thái. Hiện nay, có nhiều cách tiếp cận trong việc xác định phân loại loài, từ kỹ thuật dựa trên hình thái đến phân loại bằng chỉ thị phân tử hoặc kết hợp nhiều phương pháp, trong đó DNA barcode đã phát triển và thể hiện nhiều ưu điểm. Bốn loài nghiên cứu Paphiopedilum henryanum, Paphiopedilum coccinaum, Paphiopedilum helenae và Paphiopedilum × hermannii thuộc section chuẩn (Paphiopedilum) của phân chi Paphiopedilum, là các loài có hình thái tương đồng cao. Bài báo này trình bày kết quả phân tích hình thái thực vật của 4 loài lan Hài này, đồng thời xác định và phân tích 2 trình tự DNA barcode ITS và trnH-psbA để phân loại và nhận diện chúng. Bảng kết quả phân tích hình thái có thể sử dụng để nhận diện các loài Hài trong nghiên cứu. Kết quả phân tích DNA barcode hai chỉ thị ITS và trnH-psbA cho thấy phù hợp khi nghiên cứu phân loại lan Hài, tuy nhiên không phù hợp để nhận diện từng loài nghiên cứu ngoại trừ loài lai Paphiopedilum × hermannii.

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