NHÂN GIỐNG LAN HUỆ (Hippeastrum sp.) HOA CÁNH KÉP BẰNG PHƯƠNG PHÁP CHẺ CỦ

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Tác giả: Hữu Hùng Lương, Thị Xuân Thủy Vì

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.385-392

Bộ sưu tập: Báo, Tạp chí

ID: 339862

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Hiện nay, Lan huệ (Hippeastrum sp.) hoa cánh kép đang được người chơi hoa ưa chuộng, nhưng giống chủ yếu được nhập ngoại với giá thành cao, số lượng hạn chế. Vì vậy, nghiên cứu nhân giống vô tính có hệ số nhân nhanh, thời gian ngắn để đáp ứng được nhu cầu về giống Lan huệ cánh kép cho thị trường nước ta là một yêu cầu cấp thiết. Ngâm các mảnh chẻ củ trong BAP với nồng độ khác nhau trong thời gian 30 phút, kết quả thu được BAP thích hợp cho tái sinh chồi từ các mảnh củ chẻ bổ miếng là 2,0 mg/l, chẻ một vảy rời là 1,0 mg/l và chẻ 2/3 củ ở nồng độ 2,5 mg/l. Ngâm các mảnh chẻ củ trong BAP kết hợp với α-NAA ở nồng độ khác nhau trong thời gian 30 phút, kết quả cho thấy BAP 2,0 mg/l kết hợp với α-NAA 0,6 mg/l là thích hợp nhất cho tạo đa chồi mảnh chẻ bổ miếng, đạt 4,31 chồi/ mẫu, mảnh chẻ một vảy rời là BAP 1,0 mg/l và α-NAA 0,6mg/l đạt 3,91 chồi/ mẫu, còn chẻ 2/3 củ BAP 2,5 mg/l với α-NAA 0,6mg/l đạt 4,21 chồi/ mẫu. Phun α-NAA nồng độ khác nhau vào chồi các mảnh chẻ với tần suất 5 ngày/lần. Kết quả thu được, α-NAA 0,4 mg/l là thích hợp nhất cho ra rễ mảnh củ chẻ bổ miếng và chẻ 2/3 củ, còn chẻ một vảy rời α-NAA 0,6 mg/l là thích hợp nhất. Tribat là giá thể tốt nhất cho sinh trưởng của cây con Lan huệ cánh kép nghiên cứu.The double hippeastrum is being interested in by flower players, but the bulb supply is limited and the bulb’s price is expensive. Therefore, researching clonal propagation with a fast multiplier and short time to meet the demand for the double hippeastrum for our country is an urgent requirement. Soaking the split tubers in BAP with different concentrations for 30 minutes, results showed that BAP 2.0 mg/l was suitable for bud regeneration the slicing pieces method
the scale of bulb method was 1.0 mg/l and the slicing 2/3 bulb method was BAP 2.5 mg/l. Soaking the split tubers in BAP combined with α-NAA at different concentrations for 30 minutes, the results showed that BAP 2.0 mg/l combined with α-NAA 0.6 mg/l was suitable to creat multiple shoots of the slicing pieces method, reaching 4.31 shoots/sample
the scale of bulb method BAP 1.0 mg/l and α-NAA 0.6 mg/l was the best reaching 3.91 shoots/sample
the slicing 2/3 bulb method BAP 2.5 mg/l with α-NAA 0.6mg/l was the best reaching 4.21 shoots/sample. The shoots of the split tubers were sprayed with different concentrations of α-NAA at a frequency of every 5 days, the results showed that α-NAA 0.4 mg/l was the most suitable for rooting of slicing pieces and slicing 2/3 bulb methods
the scale of bulb method α-NAA 0.6mg/l was the most suitable. Tribat medium was the best for the growth of the double hippeastrum.

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