ĐẶC ĐIỂM GIẢI PHẪU VÀ TRÌNH TỰ CHỈ THỊ trnH-psbA PHÂN LẬP TỪ MẪU CÂY TRÀ HOA VÀNG THU THẬP TẠI THÁI NGUYÊN

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Tác giả: Manivanh Yongsa, Thị Thu Ngà Nguyễn, Khánh Linh Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.439-447

Bộ sưu tập: Metadata

ID: 339869

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Camellia sp. is rare and a valuable nutritious drink and medicinal herb. It has been considered a high economic plant. To date, the research of this genus has only been carried out on a few species, with the main focus on morphology but some camellia species have many similar morphological characteristics, so they are easily influenced and inaccurate. Therefore, there is a need to research the gene's characteristics to improve efficiency to preserve precious genetic resources. This study was conducted to analyze morphological and molecular characteristics for species identification purposes and to provide more information about a chloroplast DNA barcode on the subject of Camellia, serving species identification service to improve the efficiency and conservation of the rare and precious Camellia species in Vietnam. The results of the study have described in detail the anatomical characteristics of the stem, leaf, and petiole. Using the trnH-psbA sequence analyzing method, the sequence of the trnH-psbA gene has a size of 396 nucleotides. trnH-psbA zone similarity index on the nucleotide sequence of yellow flower tea sample compared to 10 trnH-psbA sequences on GenBank varies between 97.51% to 99.49%. As such, the trnH-psbA sequence of the yellow flower tea sample is 99.49% similar to that of Camellia tamdaoensis coded as NC 069227.1.Trà hoa vàng là loài cây quý hiếm, có nhiều giá trị sử dụng và đa tác dụng trong y học. Đây là loài cây dược liệu quý, đã và đang mang lại nhiều giá trị kinh tế cao. Hiện nay, phân tích về Trà hoa vàng chủ yếu dựa vào chỉ thị hình thái nhưng còn gặp nhiều khó khăn, bởi một số loài Trà hoa vàng có nhiều đặc điểm hình thái tương tự nhau nên dễ bị nhầm lẫn. Vì vậy, cần có các nghiên cứu đặc điểm trình tự gen để nâng cao hiệu quả và bảo tồn nguồn gen quý. Nghiên cứu này được thực hiện nhằm phân tích đặc điểm hình thái giải phẫu và phân tử phục vụ mục đích định danh loài và cung cấp thêm thông tin về một mã vạch DNA lục lạp trên đối tượng cây Trà hoa vàng, phục vụ giám định loài để nâng cao hiệu quả và bảo tồn loài Trà hoa vàng quý hiếm ở Việt Nam. Bằng phương pháp giải phẫu, kết quả nghiên cứu đã mô tả chi tiết đặc điểm cấu tạo của các bộ phận thân, lá và cuống lá. Bằng phương pháp phân tích trình tự gen của mẫu cây Trà hoa vàng, trình tự gen trnH-psbA thu được có kích thước 396 bp. So sánh với 10 trình tự trnH-psbA trên Genbank, chỉ thị trnH-psbA của mẫu thí nghiệm dao động từ 97,51% đến 99,49% và có đặc điểm về di truyền gần nhất (99,49%) với loài Camellia tamdaoensis mang mã số NC_069227.1.

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