NGHIÊN CỨU TÁCH CHIẾT SAPONIN TỔNG SỐ VÀ KHẢ NĂNG CHỐNG OXY HÓA TỪ DỊCH CHIẾT CÂY ĐINH LĂNG LÁ NHỎ (POLYSCIAS FRUTICOSA (L.) HARMS) TRỒNG TẠI THÁI NGUYÊN

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Tác giả: Thanh Ninh Lê, Thương Tuấn Nguyễn, Tiến Dũng Nguyễn, Thị Thu Hà Trần, Thị Thu Thảo Phạm Trang Nhung Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2023

Mô tả vật lý: tr.316-323

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ID: 340127

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Đinh lăng (Polyscias fruticosa (L.) Harms) thuộc họ Araliaceae, được trồng rộng rãi khắp Việt Nam và thường được dùng trong một số bài thuốc dân gian nhằm tăng cường sức khỏe cho con người. Thành phần dược liệu chính trong cây đinh lăng là các hợp chất saponin triterpenoid. Mặc dù đã có một số nghiên cứu tách chiết saponin từ lá, rễ cây đinh lăng và đánh giá tác dụng sinh học, tuy nhiên hiệu suất tách chiết phụ thuộc nhiều vào phương pháp và nguồn vật liệu. Trong nghiên cứu này, chúng tôi tiến hành khảo sát một số yếu tố như phương pháp chiết, nồng độ, tỉ lệ dung môi, thời gian và nhiệt độ ảnh hưởng đến hiệu quả tách chiết saponin tổng số từ lá cây đinh lăng thu thập tại tỉnh Thái Nguyên và đánh giá khả năng chống oxy hoá trong dịch chiết thu được. Kết quả cho thấy, điều kiện tách chiết tối ưu là sử dụng dung môi methanol, tỉ lệ nguyên liệu/dung môi là 1:12, tách chiết trong vòng 4 giờ tại nhiệt độ 70℃ bằng phương pháp khuấy trộn gia nhiệt. Dịch chiết lá được xác định bằng phương pháp quang phổ, hàm lượng saponin là 8,12 ± 0,59 mg/ml có khả năng kháng oxy hóa cao, giá trị IC50 của dịch chiết saponin đạt 17,14 μg/ml.Polyscias fruticosa (L.) Harms) belongs to the Araliaceae family and is widely grown in Vietnam and is often used sofar to promote human health. The main medicinal components in P. fruticosa are triterpenoid saponins. Although there were some studies on extraction of saponins from leaves and roots of P. fruticosa and evaluated the biological effects, the saponin extraction efficiency is highly dependent on the method and the source material. In this study, we investigated some factors such as solvent concentration, solvent ratio, time and temperature affecting the extraction efficiency of total saponins from P. Fruticosa planted in Thai Nguyen province and evaluation of antioxidant capacity of the extracts. The results show that: The optimal extraction condition is methanol solvent, the material/solvent ratio is 1:12, extraction within 4 hours at 70℃ by heating stirring method. The leaf extract was determined by spectrophotometric method, with saponin content of 8.12 ± 0.59 mg/ml. The IC50 value of the saponin extract from was 17.14 μg/ml.

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