NGHIÊN CỨU NUÔI CẤY MÔ CÂY NƯA (TACCA LEONTOPETALOIDES (L.) KUNTZE) TỪ ĐỈNH SINH TRƯỞNG

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Tác giả: Bốn Phạm Văn, Nguyễn Thị Linh, Phạm Thị Mận, Vũ Thị Thu Thanh, Trương Thị Thùy Trang, Tuấn Ninh Văn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học - Đại học Thủ Dầu Một, 2023

Mô tả vật lý: tr.22-32

Bộ sưu tập: Metadata

ID: 340989

Thêm vào giỏ Liên kết toàn văn

Cultured material were 2-week apex buds of Tacca leontopetaloides (L.) Kuntze. Using javel solution (3.5-6.5%) to sterilized the culture samples. Shoot regeneration medium was MS added BA (0.5-2.0mg/l) and NAA (0.1-0.3mg/l) simultaneously. Rooting medium was ½ MS supplemented with IBA (0.0-1.0mg/l) or NAA separately (0.0-1.0mg/l). Research results showed that, sterilized apex bud samples in javel solution at a concentration of 6.5% for 15 minutes gave the best results, the percentage of sterilized samples is 62.2% and all of them are budding
MS medium added BA 1.5mg/l and NAA 0.2mg/l simultaneously gave the highest number of shoots (4.1 shoots/cluster, effective shoots was 3.7 shoots/cluster). Best rooting medium ½ MS associated with IBA 1.0mg/l gave 95.6% rooting shoots, the number of roots was 2.9 roots/bud and the root length was 5cm
The medium consisting of 50% topsoil and 50% coconut fiber gave a survival rate of 69.4% and mean shoot height of 5.6cm after 6 weeks. The research results indicated the prospect for the production of T. leontopetaloides seedlings in-vitro. However, it is necessary to evaluate the adaptability, growth and yield of the tissue seedlings in field before deciding to produce tissue seedlings industrially.Vật liệu nuôi cấy ban đầu là chồi đỉnh được lấy sau khi nảy mầm khoảng 2 tuần. Sử dụng dung dịch javel (3,5-6,5%) để khử trùng mẫu. Môi trường nuôi cấy cơ bản là MS và ½ MS. Môi trường nhân chồi là MS được bổ sung đồng thời BA (0,5-2,0mg/l) và NAA (0,1-0,3mg/l). Môi trường ra rễ là ½ MS bổ sung IBA (0,0-1,0mg/l) hoặc NAA (0,0-1,0mg/l). Kết quả cho thấy, mẫu được xử lý bằng dung dịch javel 6,5% trong 15 phút cho kết quả tốt nhất, tỷ lệ mẫu sạch đạt 62,2% và tất cả mẫu sạch đều bật chồi
Môi trường MS + BA 1,5mg/l + NAA 0,2mg/l cho số lượng chồi cao nhất (4,1 chồi/cụm, chồi hữu hiệu là 3,7 chồi/cụm). Môi trường ra rễ tốt nhất là ½ MS + IBA 1,0mg/l cho 95,6% chồi ra rễ, số rễ là 2,9 rễ/chồi và chiều dài rễ là 5,0cm
Giá thể gồm 50% đất tầng mặt + 50% xơ dừa cho tỷ lệ sống của cây Nưa mô ở vườn ươm là 69,4% sau 6 tuần cấy. Kết quả nghiên cứu cho thấy triển vọng tốt để sản xuất cây giống Nưa bằng phương pháp nuôi cây mô. Tuy vậy, cần đánh giá khả năng thích ứng, sinh trưởng và năng suất của cây Nưa mô ngoài ruộng trước khi sản xuất cây giống mô công nghiệp.

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