Khả năng sinh miễn dịch và bảo hộ lâm sàng của vacxin chế tạo từ chủng Porcine Circovirus type 2 phân lập từ heo tại Việt Nam

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Tác giả: Thị Thu Phương Lê, Ngọc Hải Nguyễn, Ngọc Hồng Phúc Nguyễn, Tấn Liêm Nguyễn, Thị Thu Hồng Nguyễn, Hào Quang Phạm, Xuân Hạnh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.14

Bộ sưu tập: Metadata

ID: 341211

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu này nhằm đánh giá khả năng sinh miễn dịch và bảo hộ lâm sàng của vacxin vô hoạt nhũ dầu từ chủng NAVET-DongNai2/2009 (PCV2b) trên heo. Heo được tiêm vacxin chế tạo từ chủng NAVET-DongNai2/2009 bắt đầu có sự chuyển đổi kháng thể ở ngày thứ 14 sau khi tiêm.Hiệu giá kháng thể tổng cộng và kháng thể trung hòa ở nhóm heo được tiêm vacxin tương ứng là 10,31±0,52log2 và 7,00±0,89log2
cao hơn rất nhiều so với nhóm đối chứng 5,14±0,58log2 và 0log2 (P<
0,05) tại thời điểm ngày thứ 28 sau khi tiêm. Sau khi công cường độc, nhóm heo được tiêm vacxin đều khỏe mạnh, tăng trọng bình thường, có thời gian virus huyết ngắn, bài thải virus qua dịch mũi và phân với tỷ lệ thấp hơn so với nhóm heo đối chứng. Tỷ lệ heo mắc PMWS trên lô được tiêm vacxin là 0/6 và lô đối chứng là 1/4. Kết quả nghiên cứu cho thấy vacxin vô hoạt nhũ dầu này có khả năng kích thích đáp ứng miễn dịch cũng như bảo hộ heo về mặt lâm sàng chống lại virus PCV2 genotype 2b.The objective of this study aimed at determining the immunogenicity and clinical protection of the inactivated oil emulsion PCV2 vaccine produced from NAVET-ongNai2/2009 (PCV2b) virus strain in the pigs vaccinated with this vaccine. The studied result showed that seroconversion of pig started at the 14th day after injection (dpi). The titers of total antibodies and neutralizing antibodies in the vaccinated pig group were much higher than in the control group at the 28th day after injection: 10.31 ± 0.52log2 and 7.00 ± 0.89log2vs 5.14 ± 0,58log2 and 0log2 (P<
0.05), respectively. The vaccinated pigs had no increase in body temperature, without any clinical signs during the experiment. After challenge the vaccinated pigs reduced PCV2 load in the blood, lymphoid tissues, nasal and rectal swabs compared to that of the control pigs. The proportion of the vaccinated and control pig groups suffered with PMWS were 0/6 and 1/4, respectively. The studied results indicated that this inactivated oil emulsion vaccine induced immunological responses in the vaccinated pigs that appeared to provide clinical protection against PCV2b infection.

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