NGHIÊN CỨU VI NHÂN GIỐNG CÂY TRẦU BÀ ĐẾ VƯƠNG ĐỎ (Philodendron ‘Imperial Red’)

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Tác giả: Đình Biên Lại, Thị Vân Anh Nguyễn, Ngọc Trâm Phạm, Kim Chi Trần, Trọng Tuấn Trần, Thị Hương Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2023

Mô tả vật lý: tr.23

Bộ sưu tập: Báo, Tạp chí

ID: 341248

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This study performed the micropropagation procedure of Philodendron 'Imperial Red' derived from in vitro shoot. The results showed that MS medium supplemented with 1.5 mg/L benzyl adenine (BA) was suitable for the shoot multiplication stage
the number of newly formed shoots reached 6.50 shoots/explant after six weeks of culture. In the next stage, in vitro shoots were transferred to MS medium supplemented with 0.5 mg/L indole-3-butyric acids (IBA) for rooting. After eight weeks of culture in in vitro conditions, 100% of plantlets were rooted, with the average number of roots reaching 14.55, the length reaching 4.38 cm and the number of leaves reaching 6.83. The plantlets then adapted well when planted in nursery conditions, with a survival rate of 100%, average plant height of 6.80 cm, leaf length of 5.27 cm, leaf width of 2.90 cm and SPAD index of 11.68 after four weeks of planting. The number of stomata and SPAD index of plants at different growing stages were also collected in this study. The number of stomata increases gradually in the order of stages: plants grown in vitro, plants grown in nursery, and plants grown under natural conditions (mature plants). The SPAD index also changed, in increasing order: plants grown in the nursery, plants grown in vitro, and plants grown under natural conditions (mature plants). The obtained results of the study contribute to the development of a micropropagation process for this plant with high propagation coefficient and good quality seedlings, providing seedlings for the floricultural trade in Vietnam.Trong nghiên cứu này, quy trình vi nhân giống cây trầu bà đế vương đỏ (Philodendron ‘Imperial Red’) từ mẫu chồi nuôi cấy in vitro được thực hiện. Kết quả thu được cho thấy, môi trường MS (Muraghige và Skoog) bổ sung 1,5 mg/L benzyl adenine (BA) là môi trường thích hợp cho giai đoạn nhân nhanh, với số lượng chồi mới tạo thành 6,50 chồi/mẫu sau 6 tuần nuôi cấy. Trong giai đoạn tiếp theo, chồi được chuyển sang môi trường MS có bổ sung 0,5 mg/L indole-3-butyric aicd (IBA) để tạo rễ. Sau 8 tuần nuôi cấy ở điều kiện in vitro, tỷ lệ cây con ra rễ 100% với trung bình 14,55 rễ/mẫu, chiều dài đạt 4,38 cm và số lá đạt 6,83. Các cây con sau đó thích nghi tốt khi được đưa ra trồng ở điều kiện vườn ươm, với tỷ lệ sống 100%, trung bình chiều cao cây 6,80 cm, chiều dài lá 5,27 cm, chiều rộng lá 2,90 cm và chỉ số diệp lục (SPAD) là 11,68 sau 4 tuần trồng. Khí khổng và chỉ số SPAD của cây ở các giai đoạn nuôi trồng khác nhau cũng được quan sát ở nghiên cứu này. Số lượng khí khổng gia tăng dần theo thứ tự các giai đoạn: cây nuôi cấy ở giai đoạn in vitro, cây trồng ở vườn ươm, và cây được trồng ở điều kiện tự nhiên (cây thành thục). Chỉ số SPAD cũng có sự thay đổi, theo thứ tự gia tăng dần là: cây trồng ở vườn ươm, cây nuôi cấy ở giai đoạn in vitro, và cây được trồng ở điều kiện tự nhiên (cây thành thục). Các kết quả đạt được của nghiên cứu góp phần xây dựng một quy trình vi nhân giống loài cây này có hệ số nhân giống cao, chất lượng cây giống tốt, cung cấp cây giống cho thị trường cây cảnh ở Việt Nam.

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