EFFECTS OF ULTRASOUND-ASSISTED EXTRACTION CONDITIONS ON THE RECOVERY OF TRITERPENOIDS AND ANTIOXIDANT ACTIVITY OF Curculigo orchioides Gaertn RHIZOMES

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Tác giả: Dang Thi Cuong, Thi Huu Hanh Pham, Van Man Phan, Chi Hai Tran

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2023

Mô tả vật lý: tr.127

Bộ sưu tập: Metadata

ID: 341271

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Mục tiêu của nghiên cứu này là nghiên cứu ảnh hưởng của sự hỗ trợ sóng siêu âm đến quá trích ly để thu hồi triterpenoids và hoạt tính kháng oxy hóa của rễ sâm cau C. orchioides sử dụng phương pháp bề mặt đáp ứng (RSM). Những thông số của quá trình trích ly có sự hỗ trợ của siêu âm (UAE) trong nghiên cứu đã được khảo sát gồm nhiệt độ trích ly (30 - 60°C), công suất siêu âm (150 - 350 W), và thời gian siêu âm (5 - 45 phút). Mô hình bề mặt bậc hai đã được thiết lập và xử lý thống kê được sử dụng để đánh giá mô hình. Kết quả cho thấy hiệu suất thu nhận triterpenoids và hoạt tính kháng oxy hóa (khả năng bắt gốc tự do ABTS) bị ảnh hưởng đáng kể bởi nhiệt độ và thời gian (p<
0.05). Điều kiện tối ưu cho quá trình thu hồi triterpenoids và ABTS là nhiệt độ 50,63oC, công suất 322,5W và thời gian siêu âm 44,9 phút. Tại điều kiện này, kết quả thực nghiệm tương tự với kết quả dự đoán trên mô hình. So với phương pháp trích ly truyền thống thì phương pháp UAE cho giá trị hàm lượng triterpenoid và ABTS cao hơn. Khi phân tích GC-MS, dịch chiết có 6 hợp chất có khả năng kháng oxy hóa. Điều này có thể cho thấy phương pháp UAE là một phương pháp đầy hứa hẹn, hiệu quả và kỹ thuật xanh để trích ly các hợp chất có hoạt tính sinh học từ rễ sâm cau C. orchioides.>
The objective of this study is to investigate the effects of ultrasound-assisted extraction (UAE) conditions on the recovery of triterpenoids and antioxidant activity of C. orchioides rhizomes using response surface methodology (RSM). The UAE parameters under study included extraction temperature (30 - 60°C), ultrasonic power (150 - 350 W), and extraction time (5-45 min). The quadratic response models were generated, and statistical analysis was performed to validate the models. The results showed that the recovery of triterpenoids and antioxidant activity (ABTS radical scavenging activity) were significantly affected by temperature and time process (p<
0.05). The optimal conditions for the recovery of triterpenoids and ABTS were 44.90 min, 50.63 °C, and ultrasonic power of 322.50 W. Under these conditions, the experimental results agreed with the predicted values. The UAE extraction technique was found to be more efficient in the extraction of triterpenoids and antioxidant capacity in comparison with the conventional extraction method (SE). Furthermore, GC-MS analysis of optimized extract showed the presence of six components in the extracts which related to the antioxidant activity. It could be suggested that the UAE method is a promising, efficient, and green technology for the extraction of bioactive compounds from C. orchioides rhizomes>

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