EFFECTS OF ULTRASOUND, MICROWAVE ON SAPONINS, POLYPHENOLS EXTRACTION FROM Musa balbisiana FRUIT AND XANTHINE OXIDASE INHIBITION ACTIVITY OF THE OBTAINED EXTRACT

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Tác giả: Dinh Khanh Dieu, Thi Ngoc Nhon Hoang

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm (Tên mới: Tạp chí Khoa học Đại học Công Thương), 2023

Mô tả vật lý: tr.158

Bộ sưu tập: Metadata

ID: 341274

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Polyphenol và saponin là các hợp chất hoạt tính sinh học quan trọng có nguồn gốc từ thực vật với nhiều lợi ích sức khỏe khác nhau, đã thu hút sự chú ý của các nhà nghiên cứu nhằm khai thác chúng từ nhiều nguồn khác nhau. Trong nghiên cứu này, ảnh hưởng sóng siêu âm và vi sóng đến quá trình trích ly polyphenol và saponin từ quả chuối hột được nghiên cứu. Tiến hành tối ưu điều kiện trích ly bằng phương pháp bề mặt đáp ứng (RSM) và đánh giá hoạt tính kháng enzyme xanthine oxidase của dịch chiết thu nhận được. Kết quả cho thấy phương pháp trích ly có hỗ trợ siêu âm cho hiệu suất trích ly polyphenol và saponin từ trái chuối hột cao hơn so với phương pháp trích ly có hỗ trợ vi sóng. Các điều kiện trích ly tối ưu có hỗ trợ siêu âm qua phương pháp bề mặt đáp ứng (RSM) được xác định là công suất 233,85 W trong 15,78 phút thu được hàm lượng polyphenol và saponin lần lượt là 59,07 mgGAE/mgdm và 48,98 mg/mgdm. Hoạt tính ức chế enzyme xanthine oxidase cao với giá trị IC50 = 281,94 μg/mL, chứng tỏ tiềm năng chống bệnh gút cao của dịch chiết thu được.Polyphenols and saponins are crucial bioactive compounds derived from plants with various health benefits, which have attracted researchers' attention for a long time to find them from different sources. In this study, the effects of ultrasonic and microwave treatment on the polyphenols and saponins extraction from M. balbisiana fruit were investigated. The response surface methodology (RSM) method was selected to optimize the extraction conditions. The anti-xanthine oxidase enzyme activity of the obtained extracts was also determined. The results show that the ultrasonic treatment has higher extraction efficiency of polyphenols and saponins than the microwave one. The obtained conditions via the RSM method were determined at 238.35 W for 15.78 min resulting in polyphenols, and saponins contents of 59.07 mgGAE/mgdm and 48.98 mg/mgdm, respectively. The anti-xanthine oxidase inhibition activity was high with IC50 = 281.94 μg/mL, demonstrating the high anti-gout potential of the obtained extract

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