This research synthesizes the most advanced progress on biodiversity in recent decades to develop a set of information about the current condition of biodiversity in the Pu Mat national park. Data was collected from reliable documents nationally, updated statistics, and maps from management committees associated with field surveys in the study area. After implementing data synthesis, analysis, and inheritance from the previous related research and using a geographic information system (GIS), the findings indicated that: 1) Pu Mat National Park protects the rare genetic resources of Vietnam, which include 2600 floral species, 1121 animal species, and four typical ecosystems of evergreen- broadleaf natural forest, mixed wood-bamboo ecosystem, natural bamboo ecosystem and grassshrubs ecosystem
2) establishing an ecosystem map based on the integration of the forest current maps, forest inventory maps, land use current maps in Pu Mat National Park area, scale 1:50,000
and 3) identifying factors challenging in biodiversity conversation in Pu Mat national park. The main challenging factors present, (1) the need of using biodiversity resources
(2) the urbanization process
(3) the interference with the environment and habitat from human beings
(4) climate change
and (5) the development of tourism. Understanding the current state of the study area and gaining a comprehensive understanding of biodiversity would allow for the most feasible and practical conservation strategies to be suggested for Pu Mat National Park.