SÀNG LỌC VÀ ĐÁNH GIÁ HOẠT TÍNH ENZYME THỦY PHÂN ACETYL ESTERASE VÀ FERULOYL ESTERASE TỪ MỘT SỐ CHỦNG NẤM PHÂN LẬP TẠI RỪNG MƯA NHIỆT ĐỚI PHÍA BẮC

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Tác giả: Đình Giáp Vũ

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học & Công nghệ - Trường Đại học Công nghiệp Hà Nội, 2023

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ID: 342662

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Lignocellulose is the biomass source that has a low commercial value, but it is economically important when used as a raw material for applications in biotechnology. Fungi are known to be able to biosynthesize various hydrolytic enzymes to effectively attack lignocellulose structures. Carbohydrate esterase (CE) hydrolysis enzyme is of role importance in the hydrolysis of the lignocelluloses, which represents a group of hydrolases enzyme for the separation or formation of saturated and aromatic esters. In which, acetyl esterase (AE) and feruloyl esterase (FAE) break down branch chains of the cell wall polysaccharide structure to cut the interaction between xylan chains and xylan with lignin to separate lignin from the lignocellulosic structure. In this study, 23 fungal strains which isolated in the Northern Tropical Rain Forest (Cuc Phuong National Park) were screened for FAE and AE enzyme activity. In which, 20/23 fungal strains have been identified exhibit activity from 01 to 02 enzymes. The strains showed the highest AE and FAE activity were identified as Lentinus brumalis GNB10 (≈ 2353.8 U/L), and Xylaria sp. GNB3 (D = 20mm), respectively.Lignocellulose là nguồn sinh khối có giá trị thương mại thấp nhưng có vai trò quan trọng về mặtkinh tế khi được sử dụng cho các ứng dụng trong công nghệ sinh học. Nấm được biết đến có khả năngsinh tổng hợp nhiều enzyme thủy phân khác nhau để tấn công hiệu quả các cấu trúc lignocellulose trongthành tế bào thực vật. Carbohydrate esterase (CE), đại diện cho một nhóm lớn các hydrolase xúc tác sựphân tách hoặc hình thành các ester no và ester thơm. Trong đó, hai enzyme acetyl esterase (AE) vàferuloyl esterase (FAE) hoạt động trên các chuỗi nhánh của cấu trúc polysaccharide để phân cắt liên kếtcầu nối giữa các chuỗi xylan và giữa xylan với lignin để tách riêng phần lignin ra khỏi cấu trúc lignocelluloses. Trong nghiên cứu này, 23 chủng nấm phân lập tại rừng mưa nhiệt đới phía Bắc (Vườnquốc gia Cúc Phương) được sàng lọc hoạt tính enzyme FAE và AE. Trong đó, đã xác định được 20/23chủng biểu hiện hoạt tính từ 01 đến 02 enzyme. Chủng biểu hiện hoạt tính enzyme AE và FAE cao nhấttương ứng là Lentinus brumalis GNB10 (≈ 2.353,8U/L), và Xylaria sp. GNB3 (D = 20mm).

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