Khảo sát một số hoạt tính sinh học in vitro từ dịch chiết lá cây thạch vĩ (Pyrrosia lingua (Thunb.) Farwell)

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Tác giả: Thế Vinh Bùi, Thị Thu Hương Nguyễn, Ngọc Khôi Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Y Dược học Phạm Ngọc Thạch, 2024

Mô tả vật lý: tr.130

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ID: 344309

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Lá cây thạch vĩ (Pyrrosia lingua (Thunb.) Farwell) được biết đến như là một loại thảo dược có tác dụng lợi tiểu, tiêu viêm, hỗ trợ điều trị sỏi thận, viêm bàng quang, viêm tiết niệu, niệu đạo. Trong bối cảnh Việt Nam vẫn chưa có nhiều nghiên cứu về lá cây thạch vĩ, đề tài này được thực hiện nhằm khảo sát một số hoạt tính sinh học in vitro như kháng oxy hóa, kháng khuẩn, kháng nấm từ dịch chiết toàn phần từ lá cây thạch vĩ. Lá cây thạch vĩ được tách chiết bằng phương pháp ngấm kiệt theo một điều kiện tách chiết ngẫu nhiên (dung môi ethanol 70%, tỷ lệ nguyên liệu: dung môi là 1:10 (g/ml), 60 phút, 50ºC) để thu được cao chiết toàn phần. Cao chiết sau đó được pha loãng thành dịch chiết toàn phần. Sử dụng dịch chiết toàn phần này để khảo sát tiếp khả năng kháng oxy hóa (dựa vào khả năng khử gốc tự do của 2,2 - diphenyl - 1 - picrylhydrazyl) và khả năng kháng khuẩn, kháng nấm (bằng phương pháp đặt đĩa kháng sinh). Dịch chiết từ lá cây thạch vĩ có khả năng kháng oxy hóa cao (IC50 = 76,96 mg/ml), nhưng vẫn thấp hơn vitamin C (IC50 = 51,86 mg/ml) là mẫu đối chứng. Dịch chiết có khả năng kháng lại một số chủng vi khuẩn (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae) ở nhiều nồng độ khảo sát (60 - 100 mg/ml). Ở nồng độ dịch chiết càng cao thì hoạt tính kháng khuẩn càng tăng dần. Tuy nhiên, dịch chiết không có khả năng kháng được vi khuẩn Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa và vi nấm Candida albican sở tất cả các nồng độ khảo sát. Nghiên cứu này đã khảo sát được một số hoạt tính sinh học in vitro của dịch chiết từ lá cây thạch vĩ giúp bổ sung tiềm năng chữa bệnh từ lá cây thạch vĩ, từ đó làm cơ sở cho các nghiên cứu sâu hơn về hoạt tính sinh học khác của lá cây thạch vĩ.Abstract Tongue fern Pyrrosia lingua (Thunb.) Farwell is known as an herbwith diuretic, anti - inflammatory, and supportive treatment for kidney stones, cystitis, urethritis, and urethral inflammation. In Vietnam, there are still not many studies on the leaves of tongue fern, the aim of this study is to study some in vitro biological activities such as antioxidant, antibacterial, and antifungal activities of whole extracts fromtongue fern. Tongue fern were extracted by random extraction conditions (ethanol 70%, raw materials: ethanol (1:10 g/ml), 60 minutes, 50ºC). The extract was then diluted to solution. Use this solution to study the antioxidant activities (based on the ability to eliminate free radicals of 2,2 - diphenyl - 1 - picrylhydrazyl)
antibacterial and antifungal activities (by antibiotic disc set). This study evaluated the ability to capture free radicals DPPH of tongue fern with IC50 value (76,96 mg/ml), whereas vitamin C (51,86 mg/ml). In this study, whole extracts capable of inhibiting the expression of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae (60 - 100 mg/ml). At the higher concentration of the extract, the antibacterial activities increase gradually. However, this extract does not have the ability to resist Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicansat all investigated concentrations. The results of this study help some biological activities of tongue fern extracts were investigated for supplement therapeutic potentials for further studies on other biological activities. DOI: 10.59715/pntjmp.2.3.16

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