Nghiên cứu đặc điểm vi học và khảo sát một số yếu tố ảnh hưởng hàm lượng flavonoid chiết xuất từ lá cây nhội (Bischofia javanica Blume) tại Lâm Đồng

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2023

Mô tả vật lý: tr.259-271

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ID: 345292

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In recent years, the use of natural herbal remedies in disease treatment in Vietnam has been significantly considered. Research on the chemical composition, microscopic characteristics, and extraction of active components from herbal materials play an important role in medicinal applications. This study aims to describe the microscopic features of fresh Bishopwood leaves using a dual staining method, examine leaf powder under a microscope, determine the components using chemical reactions, and investigate the influence of factors such as ethanol concentration, time, temperature, and the ratio of raw materials to solvents on the process of extracting flavonoids from Bishopwood leaves. Through unique chemical reactions, the components of Bishopwood leaves were identified to include flavonoids, phenolics, tannins, steroids, proteins, carbohydrates, and anthranoids. Microscopic examination of fresh Bishopwood leaves and leaf powder under a microscope revealed characteristic structural components including epidermis, stomata, parenchyma, sclerenchyma, fiber bundles, and vascular bundles. The appropriate conditions for extracting flavonoids from Bishopwood leaves were using 50% ethanol as the extraction solvent, a 3-hour extraction time, a temperature of 60 °C, and a raw material-to-solvent ratio of 1 : 30 (g/mL). The research findings serve as valuable information contributing to the identification, standardization, and development of new drugs from local medicinal materials.Trong những năm gần đây, việc sử dụng thảo dược tự nhiên trong điều trị bệnh tại Việt Nam rất được quan tâm. Việc nghiên cứu thành phần hóa học, đặc điểm vi học và chiết xuất các thành phần có hoạt tính từ nguồn nguyên liệu đóng vai trò quan trọng trong ứng dụng điều trị bệnh. Nghiên cứu này tiến hành mô tả đặc điểm vi phẫu của lá nhội tươi bằng phương pháp nhuộm kép, soi bột lá dưới kính hiển vi, sơ bộ xác định thành phần hóa học bằng phản ứng hóa học, khảo sát ảnh hưởng của các yếu tố nồng độ ethanol, thời gian, nhiệt độ và tỉ lệ nguyên liệu/ dung môi đến quy trình chiết xuất flavonoid từ lá nhội. Bằng các phản ứng hóa học đặc trưng, sơ bộ xác định thành phần của lá nhội gồm có các nhóm chất flavonoid, phenolic, tannin, steroid, protein, carbohydrat và anthranoid. Vi phẫu lá nhội tươi và soi bột lá nhội dưới kính hiển vi xác định được các thành phần cấu trúc đặc trưng gồm biểu bì, lỗ khí, mô dày, mô mềm, mô cứng, bó libe - gỗ và các mảnh mạch. Điều kiện phù hợp để chiết xuất flavonoid từ lá cây nhội bao gồm dung môi chiết xuất là ethanol 50%, thời gian chiết xuất 3 giờ, nhiệt độ 60 °C và tỉ lệ nguyên liệu/ dung môi 1 : 30 g/mL. Kết quả nghiên cứu là nguồn thông tin quan trọng góp phần nhận biết, tiêu chuẩn hóa và phát triển thuốc mới từ nguồn dược liệu địa phương.

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