Thực trạng ô nhiễm vi sinh vật bề mặt bên trong vòi chiết rót nước thành phẩm tại các cơ sở sản xuất nước uống đóng bình ở Khánh Hòa

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2023

Mô tả vật lý: tr.295-303

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ID: 345295

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The finished water filling tap is the final contact point of filtered drinking water before bottling. Therefore, microbial contamination at this site may result in cross-contamination into the finished water in the bottle. This study was conducted to evaluate microbiological contamination on the inner surface of the finished water filling tap at the bottled drinking water producers in Khanh Hoa in 2022. A total of 93 swab samples of the inside surface of the tap at 93 producers were subjected to a test for ATP (Adenosine Triphosphate), total plate count (TPC), coliform, Escherichia coli, Pseudomonas aeruginosa, Streptococci feacal, and Clostridia. There were 72/93 (77.4%) samples with ATP values higher than 300 RLU/cm2. The geometric mean of ATP and TPC were 3.1±0.8 log (RLU/cm2) and 3.5±1.2 log (CFU/cm2), respectively. The ATP and TPC were linear correlated, indicating that the surface ATP value inside the finished water filling tap was mainly formed by microorganisms. In addition, 6/93 (6.5%) samples were found to be positive for coliform, including 3 strains of Enterobacter cloacae, 2 strains of Klebsiella pneumoniae and 1 strain of Enterobacter aerogenes. Moreover, 1/93 (1.1%) samples were infected with P. aeruginosa. All 7 strains of these bacteria were biofilm producers under laboratory conditions. Our results demonstrated that the cleaning and decontamination of finished water filling taps were not properly processed, thereby increasing the risk of microbial contamination into finished bottled water.Vòi chiết rót là nơi tiếp xúc cuối cùng của nước thành phẩm trước khi nước được đóng chai, đóng bình. Vì vậy, ô nhiễm vi sinh vật tại vị trí này có thể gây nhiễm chéo vào nước thành phẩm. Nghiên cứu được thực hiện nhằm mô tả thực trạng ô nhiễm vi sinh bề mặt bên trong vòi chiết rót nước thành phẩm tại các cơ sở sản xuất nước uống đóng bình ở Khánh Hòa năm 2022. Tổng số 93 mẫu swab bề mặt bên trong vòi chiết rót tại 93 cơ sở được xét nghiệm chỉ số ATP (Adenosine Triphosphate), tổng số vi sinh vật hiếu khí (TPC), coliform, Escherichia coli, Pseudomonas aeruginosa, Streptococci feacal và Clostridia. Kết quả phát hiện 72/93 (77,4%) mẫu có giá trị ATP cao hơn 300 RLU/cm2. Giá trị ATP và TPC trung bình lần lượt là 3,1±0,8 log (RLU/cm2) và 3,5±1,2 log (CFU/cm2). Hai chỉ số ATP và TPC có mối liên quan tuyến tính với nhau, điều này cho thấy giá trị ATP bề mặt bên trong vòi chiết rót nước thành phẩm chủ yếu do vi sinh vật tạo thành. Ngoài ra, phát hiện 6/93 (6,5%) mẫu nhiễm coliform, trong đó gồm 3 chủng là Enterobacter cloacae, 2 chủng Klebsiella pneumoniae và 1 chủng Enterobacter aerogenes. Có 1/93 (1,1%) mẫu nhiễm P. aeruginosa. Tất cả 7 chủng vi khuẩn này đều có khả năng hình thành cấu trúc màng sinh học trong điều kiện phòng thí nghiệm. Kết quả nghiên cứu cho thấy rằng công tác vệ sinh, khử nhiễm vòi chiết rót nước thành phẩm chưa được đảm bảo, từ đó làm gia tăng nguy cơ ô nhiễm vi sinh vật vào nước đóng bình thành phẩm.

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