Phân lập, tuyển chọn vi khuẩn nội sinh đối kháng với nấm Exobasidium vexans gây bệnh phồng lá chè

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Tác giả: Thị Hồng Hải Nguyễn, Thị Lý Thu Phạm, Thị Thu Hằng Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Trường Đại học Mở Hà Nội, 2023

Mô tả vật lý: tr.75

Bộ sưu tập: Metadata

ID: 346388

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Bệnh phồng lá chè do nấm Exobasidium vexans gây hại ở lá non, búp non trên cây chè. Bệnh có mức độ lan nhanh, gây ảnh hưởng nghiêm trọng tới năng suất và chất lượng chè búp. Mục tiêu của nghiên cứu là phân lập tuyển chọn vi khuẩn nội sinh đối kháng với nấm gây bệnh Exobasidium vexans như một tác nhân phòng trừ sinh học. Từ 14 chủng vi khuẩn nội sinh trong mẫu rễ, thân và lá cây chè, đã tuyển chọn được 3 chủng vi khuẩn nội sinh Y2.2, Y2.5 và Y2.7 có hoạt tính đối kháng cao với nấm gây bệnh Exobasidium vexans (kính thước vòng đối kháng từ 0,7- 1,9 cm) trong điều kiện in vitro. Sau 3 ngày đồng nuôi cấy các chủng vi khuẩn này với nấm Exobasidium cho thấy sợi nấm bị gẫy vụn và dần không phát triển được. Kết quả giải trình tự đoạn 16S rDNA của 2 chủng vi khuẩn Y2.2 và Y2.5 cho thấy chủng Y2.2 có tỉ lệ tương đồng với vi khuẩn Bacillus subtilis SMY, NCIB 3610 là 100% và chủng Y2.5 có mức tương đồng với vi khuẩn Burkholderia cepacia là 100%.Tea leaf blister disease caused by the fungus Exobasidium vexans damages young leaves and young buds on tea plants. The disease has a rapid spread, seriously affecting the yield and quality of tea buds. The objective of the study was to selectively isolate endogenous bacteria that are antagonistic to the pathogenic fungus Exobasidium vexans as a biological control agent. From 14 isolates of endophytic bacteria in samples of shoots, stems and roots of tea plants, 3 strains of endophytic bacteria Y2.2, Y2.5 and Y2.7 were selected with high antagonistic activity against the pathogenic fungus Exobasidium vexans. (optimal ring diameter from 0.7 to 1.9 cm) under invitro conditions. After 3 days of co-culture of these strains with Exobasidium, the mycelium was broken and gradually failed to grow. The results of sequencing 16S rDNA fragments of two strains of bacteria Y2.2 and Y2.5 showed that strain Y2.2 had 100% similarity with Bacillus subtilis SMY, NCIB 3610 was 100% and strain Y2.5 had a similarity rate. 100% similarity to Burkholderia cepacia.

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