Một số đặc tính sinh học của Vi khuẩn Riemerella anatipestifer chủng HAN-RA 2020 gây bệnh bại huyết ở thủy cầm phân lập tại Hưng Yên

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Tác giả: Thị Mỹ Lệ Huỳnh, Thị Ngân Mai, Minh Chiến Nguyễn, Thanh Ba Nguyễn, Thị Bích Nguyễn, Thị Thu Vân Nguyễn, Văn Giáp Nguyễn, Thị Minh Hiền Quách, Văn Khánh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.17

Bộ sưu tập: Metadata

ID: 346575

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Bệnh bại huyết do vi khuẩn Riemerella anatipestifer (RA) gây ra ở thuỷ cầm, gây thiệt hại kinh tế đáng kể cho ngành chăn nuôi vịt trên toàn thế giới do tỷ lệ chết cao và giảm tăng trọng. Nghiên cứu này được thực hiện nhằm đánh giá một số đặc tính sinh học của chủng Riemerella anatipestifer (chủng HAN-RA2020) gây bệnh bại huyết, phân lập từ vịt bệnh tại Hưng Yên năm 2020. Vi khuẩn RA chủng HAN-RA2020 phát triển tốt trên môi trường thạch sô cô la, mang đầy đủ các đặc trưng của vi khuẩn RA về tính chất nhân lên, đặc tính sinh hoá. Chủng HAN-RA2020 thuộc serotype 6, có mức tương đồng cao (100%) với các chủng vi khuẩn RA được báo cáo ở Trung Quốc các năm2015, 2017, 2019. Chủng HAN-RA2020 mẫn cảm với tetracyclin, doxycyclin, enrofloxacin
kháng với ampicillin, oxytetracyclin, norfloxacin, gentamicin, colistin sulfate. Kết quả kiểm tra độc lực cho thấy chỉ số MLD của chủng HAN-RA2020 là 109CFU/ml, gây bại huyết trên vịt với triệu chứng bệnhtích điển hình. Đồng thời, chủng HAN-RA2020 có tính sinh miễn dịch với tỷ lệ bảo hộ cao (100%), là đặc tính quan trọng cho việc lựa chọn chủng trong nghiên cứu sản xuất vacxin trong tương lai.Riemerella anatipestifer (RA) infection in waterfowl causes serious economic losses in the duck industry due to high mortality and weight gain reduction. This study aimed to evaluate some biological characteristics of the RA strain isolated in Hung Yen province from ducks in 2020 (HAN-RA2020 strain). The HAN-RA2020 strain grew well on chocolate agar medium, having all biochemical characteristics of RA. The HAN-RA2020 strain belonged to serotype 6 and had 100% of similarity level in comparison with the RA strains reported in China in 2015, 2017, and 2019. The HANRA2020 strain was susceptible with tetracycline, doxycycline, enrofloxacin
resistant to ampicillin, oxytetracycline, norfloxacin, gentamicin, colistin sulfate. The result of testing virulence of this strain showed that the minimum lethal dose of the HAN- RA2020 strain was 109 CFU/ml, causing hemolytic activity in ducks with typical lesions. Also, the HAN-RA2020 strain had immunogenicity with a protection rate of 100%, which is an important characteristic in selection for vaccine production.

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