Đáp ứng miễn dịch ở heo sau cai sữa sau tiêm 2 loại vacxin lở mồm long móng GPD50 và 3PD50

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Tác giả: Ngọc Hải Nguyễn, Thị Kiều Oanh Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.15

Bộ sưu tập: Metadata

ID: 346681

Thêm vào giỏ Liên kết toàn văn

FMD vaccines are classified into: Standard potency FMD vaccine (3PD50) and high immune response vaccine (6PD50). The high immune response vaccine (6PD50) can improve the protective effect against other FMD virus strains with different topotypes. Realtime RT-PCR technique is the standard technique, commonly used in testing and diagnosing FMD. Based on the quantitative principle of realtime RT-PCR technique, the study analyzed the Ct index of realtime RT-PCR testing results for two popular commercial FMD vaccines at present in Viet Nam, such as: 6PD50 and 3PD50 vaccines. The 6PD50 vaccine had a significantly (P<
0.05) lower Ct index compared to that of the 3PD50 vaccine. However, after the first vaccine injection with either 6PD50 or 3PD50 FMD vaccine for the post weaning piglets, a very low percentage of pigs (32.5% and 40.0%) induced specific antibodies against FMD virus for both 6PD50 and 3PD50 FMD vaccines, respectively. A high maternal antibody could interfere to the efficacy of immune response after the first FMD vaccine injection for the post-weaning piglets. Both vaccines only induced immune response in pigs for protecting at a level of more than 70% after second vaccine injection in 4 weeks interval between the first and second vaccine injection, with 92% of piglets were injected with 6PD50 vaccine and 75% of piglets were injected with 3PD50 vaccine.Vacxin LMLM có 2 loại: vacxin tiêu chuẩn (3PD50) và vacxin đáp ứng miễn dịch cao (6PD50). Vacxin LMLM 6PD50 có thể nâng cao hiệu quả bảo hộ chống lại các chủng virus LMLM khác với những topotype khác nhau. Kỹ thuật realtime RT-PCR là kỹ thuật tiêu chuẩn, được sử dụng thườngquy trong xét nghiệm, chẩn đoán bệnh LMLM. Dựa trên nguyên tắc định lượng của kỹ thuật realtime RT-PCR, nghiên cứu đã phân tích chỉ số Ct của kết quả xét nghiệm realtime RT-PCR đối với 2 loại vacxin LMLM thương mại phổ biến ở Việt Nam hiện nay, đó là 6PD50 và 3PD50. Vacxin đáp ứng miễndịch cao (6PD50) có chỉ số Ct thấp hơn có ý nghĩa (P<
0,05) so với vacxin tiêu chuẩn (3PD50). Tuy nhiên sau khi tiêm mũi 1, kết quả đánh giá đáp ứng tạo kháng thể ở heo sau cai sữa của 2 loại vacxin LMLM 6PD50 và 3PD50 đã cho thấy rằng cả 2 loại vacxin 6PD50 và 3PD50 đều không tạo được miễndịch bảo hộ đạt yêu cầu theo quy định khi tỷ lệ dương tính kháng thể đặc hiệu với virus LMLM chỉ ở mức 32,5% và 40,0%
tương ứng với vacxin 6PD50 và 3PD50. Kháng thể của heo mẹ ở mức cao có thể đã ảnh hưởng đến hiệu quả đáp ứng miễn dịch ở lần tiêm vacxin mũi thứ nhất. Cả 2 loại vacxinchỉ tạo được miễn dịch bảo hộ ở mức trên 70% sau khi tiêm mũi 2, cách 4 tuần sau khi tiêm mũi 1, với tỷ lệ 92% ở heo con được tiêm vacxin 6PD50 và 75% ở heo con được tiêm vacxin 3PD50

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