Nghiên cứu thiết lập phản ứng RT-PCR và ứng dụng phát hiện sự có mặt của virus gây bệnh sốt ba ngày (BEFV) ở đàn bò nuôi tại Việt Nam

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Tác giả: Hữu Anh Đặng, Thị Mỹ Lệ Huỳnh, Văn Giáp Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.35

Bộ sưu tập: Metadata

ID: 346686

Thêm vào giỏ Liên kết toàn văn

Bệnh sốt ba ngày ở bò (three-day sickness) là một bệnh truyền nhiễm, lây qua động vật chân đốt hút máu, do Bovine ephemeral fever virus (BEFV) gây ra. Cho tới nay, BEFV đã được phát hiện ở châu Phi, châu Á, Trung Đông, châu Úc
và được nhận định có nguy cơ phát tán đến những khu vực trước đây chưa từng xuất hiện. Đứng trước nguy cơ dịch bệnh mới có thể xâm nhập bất kỳ lúc nào, rất cần thiết có sự chuẩn bị về phương pháp chẩn đoán xét nghiệm. Do đó, nghiên cứu này đã phân tích nhằm chọn được bộmồi phù hợp dùng trong phản ứng RT-PCR phát hiện BEFV. Kết quả đã xác định được 2/6 bộ mồi dùng phát hiện BEFV phù hợp nhất về trình tự nucleotide (sai khác 0%- 0,7% so với các chủng virus có trình tự công bố ở GenBank)
có giới hạn phát hiện từ 4,07 x 102 đến 8,11 x 102 bản sao bộ gen BEFV trong mẫu. Với các bộ mồi phù hợp đã được lựa chọn, có 2/152 mẫu huyết thanh bò thu thập tại Việt Nam (mẫu gộp) được xác định dương tính với BEFV.Bovine ephemeral fever (three-day sickness) is an arthropod-borne disease that affects cattle. The etiology is bovine ephemeral fever virus (BEFV). Up to date, BEFV has been known circulating in Africa, Asia, Middle East, Australia
and continues to threaten cattle populations in the previously BEFV-free areas. Facing the risk that a new disease can occur at any time, it is essential to prepare the diagnostic testing methods. Therefore, in this study, bioinformatic and experiment-based analyses in order to choose the most suitable primers used in RT-PCR for BEFV detection were carried out. As a result, 2/6 primer sets for BEFV were determined to be the most suitable in terms of nucleotide sequence match (differ 0% - 0.7%, compared to BEFV strains in GenBank). The detection limit of the two primer sets was from 4.07 x 102 to 8.11 x 102 copies. By using suitable primers, 2/152 pooled samples collected in Viet Nam were detected to be positive with BEFV.

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