Đặc điểm đa kháng kháng sinh của vi khuẩn họ Enterobacteriaceae phân lập từ cá chép cảnh (Ornamental Carp

Đặc điểm đa kháng kháng sinh của vi khuẩn họ Enterobacteriaceae phân lập từ cá chép cảnh (Ornamental Carp - KOI fish)

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Tác giả: Thành Luân Nguyễn, Thị Hải Hà Phạm, Lâm Ngọc Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2023

Mô tả vật lý: tr.59

Bộ sưu tập: Metadata

ID: 346693

Thêm vào giỏ Liên kết toàn văn

Nuôi cá cảnh hiện đang được tập trung phát triển nhanh tại thành phố Hồ Chí Minh. Tuy nhiên, việc sử dụng rộng rãi thuốc kháng sinh để phòng và điều trị bệnh động vật thủy sản nói chung và bệnh cá cảnh nói riêng đã dẫn đến sự xuất hiện các loài vi khuẩn kháng nhiều loại kháng sinh. Trong nghiên cứu này, các loài vi khuẩn họ Enterobacteriaceae phân lập từ cá chép cảnh (Ornamental carp - koi fish) có dấu hiệu mắc bệnh được đánh giá đặc điểm đa kháng kháng sinh và dự đoán vị trí gen kháng (ARGs) thông quathử nghiệm xử lý loại bỏ plasmid (plasmid curing). Dựa vào kết quả giải trình tự 16s rRNA, các chủng vi khuẩn có kiểu hình β-hemolysis (5/27) được định danh lần lượt là Vibrio cholerae (B2), Citrobacter freundii (B4), Klebsiella aerogenes (M6), Escherichiae coli (M7) và Klebsiella pneumonia (M8). Chỉ số đa kháng (MAR) với 15 nhóm và 30 loại kháng sinh khảo sát của các chủng vi khuẩn là 0,2 - 0,3. Quan trọng hơn, 6 loại kháng sinh đang sử dụng tại trang trại gồm ampicillin, erythromycin, ciprofloxacin, doxycycline, sulfamethoxazole/trimethoprim, và tetracycline đều bị kháng ít nhất bởi một chủng vi khuẩn. Đặc biệt sau khi xử lý plasmid, các chủng vi khuẩn tiếp tục thể hiện khả năng kháng với các kháng sinh được sử dụng tại trại cá, và 3/5 chủng vi khuẩn phân lập thể hiện khả năng giảm kháng kháng sinh. Tóm lại, vi khuẩn họ Enterobacteriacae phân lập được từ cá chép cảnh có thể mang cơ chế kháng kháng sinh qua nhiễm sắc thể và thông qua trung gian plasmid. Các nghiên cứu tiếp theo cần xác định các yếu tố môi trường ảnh hưởng đến khả năng di chuyển của các plasmid và các ARGs vào bộ gen, cũng như giữa các loài trong cùng hệ sinh thái để có những hiểu biết quan trọng về xu hướng kháng kháng sinh của các mầm bệnhOrnamental fish culture is rapidly expanding in Ho Chi Minh City. However, the increased use of antibiotics to prevent and cure aquatic animal disease, in general and ornamental fish disease, in particular recently has resulted in the emergence of bacteria resisting to multi-antibiotics. In this study, the Enterobacteriaceae isolated from the diseased Ornamental carp (koi fish) were assessed for multi-antibiotic resistance characteristics and guessing the position of resistant genes (ARGs) by using a plasmid curing test. Based on 16s rRNA sequencing data, the bacterial isolates with the ß-hemolysis phenotype (5/27) were identified, such as: Vibrio cholerae (B2), Citrobacter freundii (B4), Klebsiella aerogenes (M6), Escherichia coli (M7), and Klebsiella pneumonia (M8). The indicator of multi-resistance (MAR) of bacterial strains with 15 groups and 30 investigated antibiotics was 0.2 - 0.3. Especially, six antibiotics used in the farm including ampicillin, erythromycin, ciprofloxacin, doxycycline, sulfamethoxazole/trimethoprim, and tetracycline were resisted by at least one bacterial strain. After plasmid curing, the bacterial strains continue showing multi- resistance ability with antibiotics used in the fish farm, and three out of fiveisolates exhibited the reduction of antibiotic resistance. Our findings revealed that Enterobacteriaceae isolated from the Ornamental carp may carry antibiotic resistance mechanism through chromosomes and plasmids. Further study is needed to examine the environmental factors affecting to the moving ability of plasmids and ARGs into genome, as well as among the species in the same ecosystem in order to have the important understandings on antibiotic resistance trend of the pathogens.

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