Lan kim tuyến (Anoectochilus): nhân giống, nuôi trồng, hoạt tính sinh học và tiềm năng ứng dụng

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Tác giả: Duy Vũ Hoàng, Thị Luyến Nguyễn, Trương Phương Thảo Nguyễn, Văn Tịnh Nguyễn, Thị Ánh Ngọc Phan

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Thông tin xuất bản: Tạp chí Khoa học Tây Nguyên, 2023

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ID: 346782

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Anoectochilus sp is a precious medicinal plant used and exploited by many countries around the world, including Vietnam. Currently, there were many research on micropropagation, cultivation, analysis of secondary compounds and potential applications from varies differents species Anoectochilus. In this article, we summarized and updated information on optimal conditions for in vitro propagation, ex vitro, pharmacological activities and potential applications from A. roxburghii, A. setaceus, A. formosanus, A. sikkimensis, A. regalis A. lylei, A. burmannicus và A. anamensis. The in vitro results showed that Anoectochilus sp. was easily propagated on MS, supplemented with growth regulators such as BAP, Kinetine, NAA and TDZ by rapidly multiplying shoots, nodle and TCL. In addition, this orchid was susccesfully cultured with 100% survival rate by PBCM method and growth rate was optimized by coir crumbs, rice husk ash, Nitrophoska® Foliar fertilizer and neutral radiation. Furthermore, there were many secondary compounds in the spieces. They were axit stearic, axit palmitic, axit succinic, axit p-hydroxy cinnamic, o-hydroxy phenol, beta-sitosterol, daucosterol, gastrodin, gastrodigenisn, gastrodigenin and so on. In which, the main chemical components are polysaccharides, flavonoids, glycosides and kinsenosides having various potential effects to treat cough caused by pulmonary heat and tuberculosis, hemoptysis, hematuria, renal edema, snakebite, diabetes mellitus, acute hepatitis and chronic, kidney protection, rheumatoid arthritis, cancer, immunomodulation.Lan kim tuyến là loài cây thuốc quý được nhiều quốc gia trên thế giới sử dụng và khai thác trong đó có Việt Nam. Hiện nay có rất nhiều công bố nghiên cứu liên quan đến vi nhân giống, nuôi trồng, phân tích hàm lượng hợp chất thứ cấp và tiềm năng ứng dụng từ nhiều loài Lan kim tuyến khác nhau. Trong bài viết này, chúng tôi tổng hợp, cập nhật các thông tin về các điều kiện tối ưu trong quá trình nhân giống in vitro, nuôi trồng ex vitro, các hoạt tính dược lý và tiềm năng ứng dụng từ các loài: A. roxburghii, A. setaceus, A. formosanus, A. sikkimensis, A. regalis A. lylei, A. burmannicus và A. anamensis. Các kết quả nghiên cứu in vitro đã cho thấy nhân giống Lan kim tuyến bằng môi trường MS có bổ sung các chất điều hòa sinh trưởng như BAP, kinetine, NAA và TDZ bằng cách nhân nhanh chồi, đốt thân và TCL. Thêm vào đó, loài lan này đã được nuôi trồng thành công với tỷ lệ sống sót đạt 100% bằng cách sử dụng phương pháp PBCM và tỷ lệ sinh trưởng tối ưu khi sử dụng giá thể là vụn xơ dừa, tro trấu, phân Nitrophoska® Foliar và bức xạ trung tính. Ngoài ra, Anoectochilus sp. có thành phần hợp chất thứ cấp rất đa dạng như axit stearic, axit palmitic, axit succinic, axit p-hydroxy cinnamic, o-hydroxy phenol, beta-sitosterol, daucosterol, gastrodin,… và gastrodigenisn gastrodigenin. Trong đó, thành phần hóa học chính là polysacaride, flavonoid, glycoside và kinsenoside, có nhiều tiềm năng trong việc sử dụng để điều trị ho do nhiệt phổi và lao phổi, ho ra máu, đái ra máu, phù thận, rắn cắn, đái tháo đường, viêm gan cấp tính và mãn tính, bảo vệ thận, viêm khớp dạng thấp, ung thư, điều hòa miễn dịch.

1. Đọc trực tuyến

1. Đọc trực tuyến

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