Xây dựng qui trình định lượng đồng thời methylparaben và propyl-paraben trong kem bôi da bằng phương pháp sắc kí lỏng hiệu năng cao

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Tác giả: Thanh Nhàn Mai, Thị Thu Thảo Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2023

Mô tả vật lý: tr.73

Bộ sưu tập: Metadata

ID: 347418

Thêm vào giỏ Liên kết toàn văn

Các chất bảo quản methylparaben và propylparaben được dùng chủ yếu trong dược phẩm và mĩ phẩm. Tuy nhiên, việc kiểm soát các chất bảo quản là cần thiết, do các chất bảo quản này cần được sử dụng trong nồng độ cho phép để đảm bảo sự an toàn của người tiêu dùng. Nghiên cứu nhằm mục đích đưa ra qui trình định lượng đồng thời methylparaben và propylparaben trong kem bôi da bằng phương pháp sắc kí lỏng hiệu năng cao. Qui trình gồm các bước hòa tan mẫu trong ethanol:H2O (70:30, v/v) và định lượng bằng phương pháp sắc kí lỏng hiệu năng cao, sử dụng cột RP–18 (250 mm × 4,6 mm × 5 µm), đầu dò photo diode array (PDA) với bước sóng phát hiện 256 nm, pha động methanol:đệm kali dihydrophosphat (65:35, v/v). Phương pháp phân tích có độ đặc hiệu, độ tuyến tính, độ đúng và độ lặp lại đạt yêu cầu theo hướng dẫn nội dung và phương pháp của Hội nghị Hài hòa Quốc tế (International Conference on Harmonization – ICH). Phương pháp này phù hợp để phân tích các loại kem bôi da có chứa thành phần chất bảo quản methylparaben và propylparaben.The preservatives methylparaben and propylparaben are mainly used in pharmaceutical and cosmetic products. However, monitoring of these preservatives is essential as they need to be used with the allowable concentration to ensure the safety of consumers. The aim of study is to develop an analytical method for the simultaneous quantitative determination of methylparaben and propylparaben in ointments by high performance liquid chromatography. The samples are dissolved by ethanol:H2O (70:30, v/v) and quantifies by high – performance liquid chromatography, using stainless steel RP–18 column (250 mm x 4.6 mm i.d., 5 μm particle size), with a mobile phase containing methanol:kali dihydrophosphat (65:35, v/v) and PDA detector at 256 nm. This method meets requirements for specificity, system compatibility, precision, linearity and accuracy according to the ICH guideline. The extraction and measurement method could be applicable for oinments containing methylparaben and propylparaben.

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