In this paper, presenting the results of study the integrated catalyst system Mg-Al hydrotetan on the basis of -Al2O3 by co-precipitation method. The synthesized products were structurally determined by X-ray energy dispersive spectroscopy. XRD results show that Mg-Al hydrotanoxide has been successfully integrated on the γ-Al2O3 platform with a uniform degree of size grain, the peaks characterize the product clearly. The synthesized hydrotanxit/γ- Al2O3 product was used as a catalyst for the cross-esterification of the treated beef fat to produce biodiese, at 65oC within 8 hours for the amount of biodiesel to be obtained with high efficiency. The biodiesel composition after the reaction was determined by GC-MS spectroscopy, showing that the biodiesel composition is mainly methyl esters of specific fatty acids and contains few impurities.