In this study, activated carbon from durian peel (ACDP) was successfully prepared. The fabricated ACDP had a porous surface with a specific surface area of 348.0017 m²/g and an isoelectric point of 6.03. The ACDP materials were applied for adsorption of methylene blue from aqueous solution using static adsorption method. The effects of pH (3.0 – 10.0), adsorption time (30 - 210 min), the amount of adsorbent (0.01 - 0.125 g), and initial concentration of of methylene blue (50 - 500 mg/L) on the adsorption were investigated. The optimum condition was found at pH = 7.0, at adsorption time of 90 min. The maximum monolayer adsorption capacity of ACDP was 57.47 mg/g. The prepared ACDP therefore could act as a promising adsorbent for the removal of methylene blue from aqueous solution.