“Ha thu o trang” has the scientific name of Streptocaulon juventas. As a traditional medicine, “Ha thu o trang” has a bittersweet taste, acrid and cool properties, and has the effect of tonic blood, liver, and kidney. In Vietnam, “Ha thu o trang” has been known for a long time and is widely used in folk medicine to cure snakebites, premature graying of hair, clear heat, detoxify, and swelling pain. It is used as a tonic to treat depression and neurological diseases, strengthen bones and bones, live long, and blacken hair and beard. The previous studies only focused on the chemical constituents of the root of S. juventas, proving the cardenolide glycosides were the main of this species. In this study, the dried powder sample of leaves and stems vines of S. juventas was collected in Tinh Bien district, An Giang province, and hot extracted with methanol solvent to obtain the methanol extract. Dispersion of the methanol residue in water and liquid-liquid extraction with n-hexane, ethyl acetate, and n-butanol solvents, respectively, was carried out to obtain n-hexane, ethyl acetate, and n-butanol fractions. After performing column chromatography several times on the ethyl acetate fraction, four pure compounds were isolated: lupeol (1), 11-oxo-α-amyrin acetate (2), ursolic acid (3), and 3-α-O-acetyl betulinic acid (4). Their chemical structures were determined based on the analysis of 1D, 2D-NMR nuclear magnetic resonance spectroscopy data, combined with reference comparison. This is the first time that the isolation and structural determination of these compounds have been published.