Xác định cholesterol trong thực phẩm, thực phẩm chức năng bằng sắc ký khí khối phổ (GC-MS)

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2023

Mô tả vật lý: tr.352-365

Bộ sưu tập: Metadata

ID: 363473

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Cholesterol is a soft, pale yellow steroid fat that exists in the body's cell membranes and is transported in the plasma of most animals. This compound plays an important role in the physiology of the human body. However, excess cholesterol in the diet is also one of the leading causes of cardiovascular diseases. Therefore, the quantification of cholesterol in foods and functional foods is essential so that consumers can choose a diet containing the right amount of cholesterol. This study used gas chromatography-mass spectrometry to quantify cholesterol, with ISQ 7000 GC-MS system, TraceGOLD TG-5MS chromatographic column, and electron impact (EI) ionization mode. The validity of the method was confirmed according to the guidelines of the Association of Official Analytical Collaborations (AOAC). The results showed that the method has good specificity, the linearity range from 5 to 100 μg/mL in working solution, the detection limit of 1.5 to 15 mg/kg, the quantification limit of 5.0 to 50 mg/kg, depending on the matrix
the method's recovery were from 92.6 to 108.4% and the precision has relative standard deviations of 1.18 - 2.84%, meeting the requirements of AOAC. The method was used to analyze samples of dairy foods and functional foods randomly purchased in the market.Cholesterol là một chất béo steroid, thể chất mềm, màu vàng nhạt, tồn tại trong màng tế bào của cơ thể và được vận chuyển trong huyết tương của hầu hết các loại động vật. Hợp chất này có vai trò quan trọng trong sinh lý của cơ thể. Tuy nhiên, cholesterol dư thừa trong khẩu phần ăn cũng là một trong những nguyên nhân hàng đầu trong các bệnh lý về tim mạch. Vì vậy, việc định lượng cholesterol trong các loại thực phẩm, thực phẩm chức năng là rất cần thiết để người tiêu dùng có thể lựa chọn khẩu phần ăn chứa lượng cholesterol phù hợp. Nghiên cứu này sử dụng phương pháp sắc ký khí khối phổ để định lượng cholesterol, với hệ thống ISQ 7000 GC-MS, cột sắc ký TraceGOLD TG-5MS, chế độ ion hóa va chạm điện tử (EI). Giá trị sử dụng của phương pháp được xác nhận theo hướng dẫn của Hiệp hội hợp tác phân tích chính thức (AOAC). Kết quả cho thấy phương pháp có tính đặc hiệu tốt
đường chuẩn tuyến tính trong khoảng nồng độ 5-100 μg/mL của dung dịch làm việc
giới hạn phát hiện 1,5 mg/kg đến 15 mg/kg, giới hạn định lượng 5 mg/kg đến 50 mg/kg tùy theo nền mẫu
độ thu hồi của phương pháp từ 92,6 - 108,4% và độ chụm có độ lệch chuẩn tương đối 1,18 – 2,84%, đáp ứng theo yêu cầu của AOAC. Phương pháp đã được sử dụng để phân tích các mẫu thực phẩm, thực phẩm chức năng nguồn gốc từ sữa được mua ngẫu nhiên trên thị trường.

1. Đọc trực tuyến

1. Đọc trực tuyến

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