Xác định một số hormon sinh dục trong thực phẩm bảo vệ sức khoẻ bằng phương pháp sắc ký lỏng khối phổ hai lần

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Tác giả: Tạp chí Kiểm nghiệm và An toàn thực phẩm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2023

Mô tả vật lý: tr.445-457

Bộ sưu tập: Metadata

ID: 364063

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The trend of using dietary supplements in daily life is increasingly popular. However, sex hormones are illegally mixed to improve product effectiveness such as promoting hair growth, developing chest muscles, preventing skin aging, promoting growth, etc. In this study, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed and applied to quantify sex hormones: progesterone (PG), testosterone (TS), and 17β-estradiol (E2) in health supplements. A Symmetry C18 reversed-phase chromatography column (150 mm x 3.0 mm x 3.5 μm) using a mobile phase system consisting of A: 10 mM HCOONH4/0.1% HCOOH, B: ACN was used. Mass spectrometry conditions were set at positive ionization mode, precursor ion and two corresponding daughter ions were selected for each analyte. Quantification limits of PG and TS were 0.4 mg/kg and E2 was 4 mg/kg in liquid matrix, hard and soft capsules. The validated method was applied to detect sex hormones in 16 health supplement samples purchased at pharmacies in Hanoi, Vietnam and some online websites. Three samples were detected to be positive for sex hormones, including one sample containing a sex hormone (testosterone) banned from use in health supplements at a low content (about 0.5 mg/kg).Xu hướng sử dụng thực phẩm bảo vệ sức khỏe (TPBVSK) ngày càng trở nên phổ biến hơn. Tuy nhiên, các hormon sinh dục được trộn trái phép nhằm nâng cao hiệu quả sản phẩm như thúc đẩy mọc tóc, phát triển cơ ngực, ngăn ngừa lão hóa da, thúc đẩy tăng trưởng,... Trong nghiên cứu này, phương pháp sắc kí lỏng khối phổ 2 lần LC-MS/MS được phát triển và ứng dụng để định lượng các hormon sinh dục: progesteron (PG), testosteron (TS) và 17β- estradiol (E2) trong TPBVSK. Các khảo sát bao gồm điều kiện xử lý mẫu và điều kiện phân tích. Nghiên cứu sử dụng cột sắc ký pha đảo Symmetry C18 (150 mm x 3,0 mm x 3,5 μm). Điều kiện khối phổ chế độ ion hóa dương, chọn được ion mẹ và hai mảnh ion con tương ứng cho chất phân tích. Phương pháp có thể định lượng PG, TS ở hàm lượng 0,4 mg/kg và E2 ở hàm lượng 4 mg/kg trên nền viên cứng, nang mềm và dạng lỏng. Phương pháp sau khi thẩm định đã được áp dụng để phát hiện các hormon sinh dục trong 16 mẫu TPBVSK được mua tại các nhà thuốc tại Hà Nội (Việt Nam) và một số trang mạng online. Kết quả phát hiện 3 mẫu dương tính với hormon sinh dục, trong đó có một mẫu chứa hormon sinh dục bị cấm sử dụng trong TPBSVK (testosteron) theo thông tư 10/2021/TT-BYT với nồng độ rất thấp (khoảng 0,5 mg/kg).

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