In this era of Industry 4.0, the application of high technologies in the management of natural resources has become more popular and achieved certain successes. This paper presents the highly feasible applications of two technologies: (1) Big Data and (2) Cloud computing for the macrofungal resources biodiversity management, thereby building a pilot model for the management of some valuable macro mushroom resources in Vietnam. Technology 4.0 has facilitated the development of Big Data and Cloud computing techniques and applied them to increase efficiency in resources management (in general) and macrofungal biodiversity management (in particular). Specifically, the application of technological achievements helps managers accurately monitor the organism population's fluctuations, identify the harmful factors to this population, and promptly take remedial measures. In addition, management efficiency is also demonstrated through the identification of the distribution and conservation plan of precious mushroom genetic resources.