Biến động theo mùa, trọng lượng và giới tính của độc tố tetrodotoxin trong cua móng ngựa Carcinoscorpius rotundicauda thu từ biển Cần Giờ, thành phố Hồ Chí Minh, Việt Nam

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Tác giả: Thị Mai Đặng, Thị Cúc Đinh, Thị Cẩm Vân Đỗ, Đăng Thuần Trần, Hữu Quang Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học Trường Đại học Quy Nhơn, 2023

Mô tả vật lý: tr.13

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ID: 372235

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Biến động của hàm lượng tetrodotoxin (TTX) trong cua móng ngựa Carcinoscorpius rotundicauda được thu thập từ bờ biển Cần Giờ, Thành phố Hồ Chí Minh, Việt Nam từ tháng 5/2021 đến tháng 5/2022 theo mùa, trọng lượng cơ thể và giới tính được xác định bởi phương pháp sắc ký lỏng kết hợp khối phổ (LC-MS/MS). Dữ liệu cho thấy có sự thay đổi đáng kể về hàm lượng TTX theo tháng với độc tính TTX tối đa được xác định là 197,6 ± 134,5 MU/g đối với các mẫu được thu thập vào tháng 5 năm 2021, trong khi C. rotundicauda được thu thập vào tháng 1 năm 2021 chỉ đạt hàm lượng TTX trung bình 7,8 ± 8,4 MU/g. Điều thú vị là sự khác biệt về hàm lượng TTX ở các nhóm C. rotundicauda trọng lượng cơ thể khác nhau cũng có khác biệt về mặt thống kê, với độc tính TTX tối đa là 230,3 ± 116,3 MU/g được xác định cho các mẫu vật có trọng lượng cơ thể lớn hơn 300 g, trong khi mức TTX thấp nhất (9,8 ± 12,3 MU/g) được đo cho nhóm C. rotundicauda có trọng lượng cơ thể dưới 150 g. Hơn nữa, có sự khác biệt đáng kể về hàm lượng TTX giữa các nhóm C. rotundicauda cái và đực, với độc tính TTX cao hơn đáng kể được xác định cho mẫu cua móng ngựa cái (123,9 ± 45,8 MU/g) so với C. rotundicauda đực (68,7 ± 45,8 MU/g). Đặc biệt, hàm lượng TTX trung bình trong các mẫu cua móng ngựa thu ở biển Cần Giờ đạt 96,3 ± 94,2 MU/g là khá cao, cho thấy chúng không an toàn cho con người sử dụng làm nguồn thức ăn. Tuy nhiên, cua móng ngựa từ vùng biển Cần Giờ, thành phố Hồ Chí Minh, Việt Nam là nguồn tiềm năng để phân lập và tinh sạch tetrodotoxin cho các ứng dụng khác nhau (ví dụ làm thuốc).Liquid chromatography–mass spectrometry (LC-MS/MS) was used for measurement of the variation of tetrodotoxin (TTX) in a horseshoe crab Carcinoscorpius rotundicauda collected from Can Gio coast, Ho Chi Minh City, Vietnam from May 2021 to May 2022 over the seasons, body weight and gender. The data revealed that there was significant variation of TTXs level over the months with maximal TTXs toxicity determined of 197.6 ± 134.5 MU/g for specimens collected in May 2021, whereas the C. rotundicauda collected in January 2021 was found to yield minimum TTXs level of 7.8 ± 8.4 MU/g. Interestingly, the difference of TTXs level in different body-weight C. rotundicauda groups was also statistically significant, with maximal TTXs toxicity of 230.3 ± 116.3 MU/g determined for specimens having body weight of greater than 300 g, while the lowest level of TTXs (9.8 ± 12.3 MU/g) was assayed for C. rotundicauda group of body weight less than 150 g. Moreover, there was a significant difference in TTXs levels among female and male C. rotundicauda groups, with notable higher TTXs toxicity determined for female specimens (123.9 ± 45.8 MU/g) than that of male C. rotundicauda (68.7 ± 45.8 MU/g). In particular, the average level of TTXs of 96.3 ± 94.2 MU/g measured in all studied crabs indicated that they are unsafe for human consumption. Fortunately, horseshoe crab collected from Can Gio coast, Ho Chi Minh City, Vietnam is a promising source for isolation and purification of tetrodotoxin for other applications (e.g., drug).

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