TỐI ƯU HÓA QUÁ TRÌNH TRÍCH LY DỊCH CHUỐI BẰNG ENZYME THỦY PHÂN SỬ DỤNG PHƯƠNG PHÁP BỀ MẶT ĐÁP ỨNG

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Tác giả: Thị Hạnh Đỗ, Thị Dung Hà, Thế Vinh Mạc, Thị Ngọc Mai Nguyễn, Văn Sơn Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học & Công nghệ - Trường Đại học Công nghiệp Hà Nội, 2023

Mô tả vật lý: tr.138

Bộ sưu tập: Báo, Tạp chí

ID: 379498

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Mục tiêu của nghiên cứu là xác định điều kiện tối ưu của quá trình trích ly dịch chuối. Điều kiện trích ly gồm ba yếu tố được khảo sát bằng thực nghiệm là tỷ lệenzyme, nhiệt độ trích ly, thời gian trích ly. Hàm mục tiêu là hiệu suất thu hồi dịch chuối. Phương pháp bề mặt đáp ứng với mô hình Box-Behnken được sử dụng đểthiết kế thí nghiệm với tỷ lệ enzyme pectinase 0,05 - 0,07%, nhiệt độ trích ly 45 -55oC, thời gian trích ly 50 - 70 phút. Điều kiện tối ưu được dự đoán từ mô hình thực nghiệm là: tỷ lệ enzyme pectinase 0,062%, nhiệt độ trích ly 51,0oC, thời gian trích ly 65,1 phút, hiệu suất thu hồi dịch cao nhất đạt 67,77%. Thí nghiệm kiểm định kết quả tối ưu cho hiệu suất thu hồi dịch đạt 67,6%, hàm lượng phenolic tổng của mẫu dịch chiết tối ưu đạt 295,13mgGAE/L.The aim of this research was to determine the optimal condition of juice banana extraction. The extraction conditions included three factors studied by empirical research were ratio of pectinase enzyme (0.05 – 0.07%), extraction temperature (45 - 55oC) and extraction time (50 - 70 minutes). The response was the extraction yield. The response surface methodology (RSM) based on Box -Behnken was used to design experiments. The optimal conditions were predicted by using the empirical model, the extraction yield was maximised (67,77%) at ratio of pectinase enzyme of 0.062%, extraction temperature of 51.0oC, extraction time of 65.1 minutes. The optimal condition was then empirically veriöed, extraction yield was 67.6%, and the total phenolic content of optimal extract was 295.13mgGAE/L.

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