ẢNH HƯỞNG CỦA DUNG MÔI TRÍCH LY ĐẾN HÀM LƯỢNG POLYPHENOL TỔNG VÀ HOẠT TÍNH KHÁNG OXI HÓA CỦA CAO CHIẾT LÁ ỔI

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Tác giả: Thị Tuyết Nhung Đỗ, Thị Kiều Tiên Đoàn, Nguyễn Thuý Quỳnh Huỳnh, Thanh Phước Lê, Cường Quốc Nguyễn, Như Muội Nguyễn, Thị Như Ý Nguyễn, Vũ Nhật Phạm, Quang Đệ Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2024

Mô tả vật lý: tr.13 - 19

Bộ sưu tập: Báo, Tạp chí

ID: 380083

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Nghiên cứu này khảo sát ảnh hưởng của dung môi trích ly đến hàm lượng polyphenol tổng và hoạt tính kháng oxi hóa của cao chiết lá ổi Nữ hoàng. Lá ổi được thu hái tại Bạc Liêu, rửa sạch, sấy khô và nghiền thu được dạng bột mịn làm nguyên liệu cho quá trình trích ly cao. Hai loại dung môi ethanol và methanol được sử dụng để trích ly cao tổng theo phương pháp ngâm dầm. Sản phẩm cao methanol đạt hiệu suất trích ly và hàm lượng polyphenol tổng cao hơn được lựa chọn cho khảo sát các cao phân đoạn trong ba dung môi với độ phân cực khác nhau là n-hexane, dichloromethane (DC) và ethyl acetate (EA). Hoạt tính kháng oxi hóa được của các cao methanol, n-hexane, DC và EA được khảo sát theo hai phương pháp DPPH và ABTS. Kết quả cho thấy hoạt tính kháng oxi hóa của cao tổng methanol và cao EA tốt với giá trị EC50 tương ứng là 15,39 và 12,49 µg/mL. Kết quả khảo sát mở ra hướng lựa chọn dung môi phù hợp trong phát triển các sản phẩm kháng oxi hóa tự nhiên từ lá ổi Nữ hoàng.This study investigated the effect of extraction solvent on total polyphenol content and antioxidant activity of Queen guava leaf extract. The leaves were collected in Bac Lieu province, washed, dried and ground to obtain powder as raw material. Two types of solvents, namely ethanol and methanol, were used to extract by maceration method. The obtained products were determined extraction efficiency and total polyphenol content. The results showed that methanol extract acquired higher extraction efficiency and polyphenol content compared to ethanol extract. Methanol extract was selected for the investigation of fraction extracts in three solvents with different polarities: n-hexane, dichloromethane (DC) and ethyl acetate (EA). The antioxidant activity of methanol, n-hexane, DC and EA extracts was identified by DPPH and ABTS assays. The antioxidant activity of total methanol and EA extracts were the best with EC50 values of 15.39 and 12.49 µg/mL, respectively. These results facilitate selecting suitable solvent in developing natural antioxidant products from Queen guava leaf.

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