Callisia fragrans (C. fragrans) belongs to the family Commelinaceae and has wide applications in the pharmaceutical field. The leaves were extracted by distillation in 40% methanol. The volatile extract (M) was collected, while the extract remaining in the flask was fractionated with the obtained ethyl acetate extract (Y) and the remaining extract after passing ethyl acetate (B). Total polyphenols (TPCs) of extracts in (M), (Y) and (B) were determined spectrophotometrically using Folin Ciocalteu reagent, with Gallic acid used as standard. The TPCs of (M), (Y) and (B) were determined as 4.7 ± 0.22 mg GAE/g, 17.91 ± 0.0031 mg GAE/g, 47.11 ± 0.22 mg GAE/g dry weight, respectively. By liquid chromatography – mass spectrum, gallic acid and 1-O-(E)-caffeoyl-4,6-di-O-galloyl-β-D-glucopyranose were detected in (B). Furthermore, tyrosinase inhibition was detected in extract (B) by tyrosinase inhibition assay due to the highest TPC content, using kojic acid as standard due to its tyrosinase inhibitory ability. Relative inhibition of (B) was 17.29% and IC50 was 64.47 ± 0.06 µg/mL, equivalent to 2/3 the potency of kojic acid. Research has also succeeded in creating microcapsules containing TPC. The study on C. fragrans contribute to the pharmaceutical field.