In this research, a derivative of quinoline, namely 5-bromo-6-hydroxyl-3-sulfoquinol-7-yloxyacetic acid as well as one complex of Ni(II) with this ligand [Ni(QBr-2H).H2O].3H2O (NiQBr) was synthesized. The molecular formulas and structures of the complex were determined using a combination of IR, EDX, ESI MS, 1H NMR spectra and thermal analysis. In this complex, Ni(II) coordinated with QBr ligand through the oxygen atom of OH phenol, oxygen atom of OCH2 groups and the oxygen atom of carboxylate groups. The results of antimicrobial resistance testing of complex showed that NiQBr complex exhibited high activities on Bacillus subtilis and Lactobacillus fermentum with low IC50 from 0.61 - 0.71 μg/ml and NiQBr complex exhibited activities on Candida albican and Staphylococcus aureus with IC50 from 26.30 - 104.30 μg/ml.