Khảo sát hoạt tính chống oxy hóa và ức chế enzym alpha-glucosidase của Dền gai (Amaranthus spinosus L. Amaranthaceae)

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Tác giả: Bích Ngọc Giàu Lư, Phương Thanh Ngân Nguyễn, Thị Hương Giang Phạm, Thiện Vy Phan, Võ Duy Khang Phùng

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2023

Mô tả vật lý: tr.48

Bộ sưu tập: Metadata

ID: 380998

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Nghiên cứu được thực hiện nhằm làm rõ đặc điểm thực vật và cung cấp thêm thông tin về hoạt tính hạ đường huyết của Dền gai tại Việt Nam. Dền gai được định danh bằng phương pháp ADN, khảo sát đặc điểm hình thái bằng phương pháp thường quy. Sơ bộ hóa thực vật theo phương pháp Ciuley cải tiến bởi Trường Đại học Y Dược Thành phố Hồ Chí Minh. Chiết xuất cao toàn phần và 5 cao phân đoạn petroleum ether, chlorofrom, ethyl acetat, n-butanol, nước. Thử hoạt tính chống oxy hóa dựa trên mô hình 2,2-diphenyl-1-picrylhydrazyl (DPPH) và ức chế enzym α-glucosidase trên 6 mẫu cao chiết. Nghiên cứu đã xác định được loài Dền gai (Amaranthus spinosus) bằng phương pháp giải trình tự gen matK, đặc điểm hình thái tương đồng với tài liệu tham khảo. Thành phần hóa học có chứa chất béo, acid hữu cơ, flavonoid, carotenoid, triterpen, saponin, tanin, chất khử và hợp chất polyuronic. Cao phân đoạn chloroform có hoạt tính chống oxi hóa (IC50 144,97 μg/mL) và ức chế enzym α-glucosidase (IC50 239,25 μg/mL) tốt nhất. Mẫu chứng dương vitamin C (IC50 10,95 μg/mL) và acarbose (IC50 254,03 μg/mL).The research was carried out to provide more information about the characteristics and hypoglycemic activity of Amaranth in Viet Nam. Amaranthus spinosus L. identified by DNA method, and morphological characteristics were investigdted by conventional methods. Preliminary phytochemicals, according to the Ciuley method, were improved by University of Medicine and Pharmacy at Ho Chi Minh City. The total extract and five fractions of petroleum ether, chloroform, ethyl acetate, n-butanol, and water were investigated. The antioxidant activity wasanalyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay and α-glucosidase enzyme inhibition of 6 extract samples. The species was indentified as A. spinosus by sequencing the matK gene, with morphological characteristics similar to reference documents. The plant chemical composition included fats, organic acids, flavonoids, carotenoids, triterpenes, saponins, tannins, reducing agents and polyuronic compounds. Chloroform fraction extract exhibited the best antioxidant activity (IC50 144.97 μg/mL) and α-glucosidase enzyme inhibition (IC50 239.25 μg/mL), as compared to the positive controls vitamin C (IC50 10.95 μg/mL) and acarbose (IC50 254.03 μg/mL).

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