Trích ly và khảo sát hàm lượng polyphenol, hoạt tính kháng oxy hóa của dịch chiết ethanol từ hoa Hẹ (Allium ramosum L.)

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Tác giả: Gia Linh Bùi, Hải Đường Lê, Ánh Tuyết Nguyễn, Thị Trang Trần, Thị Thu Thảo Trương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2023

Mô tả vật lý: tr.66

Bộ sưu tập: Báo, Tạp chí

ID: 381002

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Nghiên cứu khảo sát ảnh hưởng của điều kiện trích ly dịch chiết ethanol từ hoa Hẹ (Allium ramosum L.), đồng thời khảo sát hàm lượng polyphenol, hoạt tính kháng oxi hoá của dịch chiết đó. Nghiên cứu này sử dụng sự hỗ trợ của sóng siêu âm để khảo sát quy trình trích ly và cho kết quả tối ưu là sử dụng dung môi ethanol 75 %, siêu âm ở nhiệt độ 50 oC trong thời gian 45 phút, đồng thời sử dụng phương pháp Folin-Ciocalteu xác định hàm lượng polyphenol và phương pháp DPPH khảo sát hoạt tính kháng oxy hóa của dịch đã chiết. Kết quả thu được đối với hàm lượng polyphenol là (51,30 ± 0,01) mgGAE/mL, đối với hoạt tính kháng oxy hóa là (0,383 ± 0,001) mg dịch chiết/mL. Ngoài ra, nghiên cứu cũng đã nhận danh được một số nhóm chất chính như phenolic, flavonoid, alkanoid trong dịch chiết bằng phương pháp hóa học. Với kết quả đạt được, ngoài việc cung cấp thêm một số thông tin khoa học về hoa Hẹ mà trước đây chưa được khám phá, kết quả nghiên cứu còn đóng góp vào việc nâng cao giá trị của một loại dược liệu quý vốn đã rất quen thuộc với người dân và góp phần làm phong phú thêm kho tàng những cây thuốc và vị thuốc cổ truyền Việt Nam.The study investigated the influence of extraction conditions on ethanol extraction from Chive flowers (Allium ramosum L.) and simultaneously examined the polyphenol content and antioxidant activity of the obtained extract. In this research, we utilized ultrasound assistance to explore the extraction process and determined that the optimal results were achieved using 75 % ethanol solvent, ultrasound at 50 oC for 45 minutes. The study successfully extracted ethanol from Chive flowers under these conditions, employing the Folin-Ciocalteu method to quantify polyphenol content and the DPPH method to assess the antioxidant activity of the extracted solution. The results revealed a polyphenol content of (51.30 ± 0.01) μgGAE/mL, and an antioxidant activity of (0.383 ± 0.001) mg extract/mL. Additionally, the study identified various chemical groups such as Phenolic, Flavonoid, and Alkaloid in the extract through chemical methods. With these findings, the research not only provides additional scientific information about Chive flowers previously undiscovered but also contributes to enhancing the value of a precious medicinal herb that has long been familiar to the people, enriching the treasure trove of Vietnamese traditional medicinal plants and remedies.

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