Objectives: To describe knowledge of cervical cancer prevention and screening and to identify associated factors among women at Thai Binh Hospital of Obstetrics and Gynecology in 2023. Participants and methods: A descriptive cross-sectional study design was conducted among 400 women aged 21-65 years at Thai Binh Hospital of Obstetrics and Gynecology to collect data on knowledge of cervical cancer prevention and screening by using a self – reported questionnaire. The maximum score for the general knowledge was 46 points. Results: The percentage of participants with good knowledge of cervical cancer prevention and screening was 38.8%. Knowledge of cervical cancer prevention and screening was related to age, occupation, and education level. Conclusion: The knowledge of cervical cancer prevention and screening among the women in the research was quite low. Therefore, it is necessary to strengthen communication and health education for women at the hospital by providing more information sheets (posters, leaflets...) about cervical cancer, vaccination and screening, therefore,they may improve their knowledge while getting the healthcare service at the hospital.