Nghiên cứu hoạt tính sinh học của vi nấm nội sinh trên cây thạch tùng javanica (Huperzia javanica)

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Tác giả: Thị Yến Hoàng, Thị Minh Thành Lê, Thanh Hà Phạm, Thị Thu Hà Trịnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Môi trường - Chuyên đề Khoa học - Công nghệ, 2024

Mô tả vật lý: tr.52-57

Bộ sưu tập: Báo, Tạp chí

ID: 384255

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The application of beneficial microorganisms as well as their secondary products in the livestock, agriculture, and food industry supply chains for sustainable green production is a developing trend. The research direction of selecting multi-activity strains to enhance the effectiveness of practical applications has been receiving attention. This study aims to select endophytic fungal strains on Huperzia javanica plant in Vietnam that have the ability to produce multi-extracellular enzymes and multi-resistance to pathogenic microorganisms by determining enzyme activity and testing antimicrobial activity. The results showed the following: (1) All 9 strains have the ability to produce 1 to 5 types of enzymes and inhibit 1 to 5 pathogenic microorganisms with potential activities
Strains TLC11 and TLC9 produce 4÷5 enzymes (cellulase, lipase, protease, phosphatase and β-galactosidase) with the highest hydrolysis zone diameters of 22÷25 mm (protease) and 20÷23 mm (lipase)
(2) Strain TLC13 inhibits all 5 tested microbial strains Escherichia coli, Staphylococcus aureus, Candida albican, Bacillus cereus, Pseudomonas aerigunosa) with the highest activity against 3 species B. cereus (24±1.2 mm), P. aerigunosa ( 26±1.1 mm) and C. albican (36±1.5 mm)
Strains TLC10 and TLC19 are resistant against 4/5 tested microorganisms except S. aureus (TLC10) and E. coli (TLC19). These strains could be a potential sources for further in-depth research aiming to expand their applications in sustainable agriculture, aquaculture, and industry production fields.Việc ứng dụng các chủng vi sinh vật (VSV) hữu ích cũng như các sản phẩm thứ cấp của chúng trong chuỗi sản xuất chăn nuôi, trồng trọt, công nghiệp thực phẩm tuần hoàn, xanh-bền vững đang được phát triển rộng rãi. Nghiên cứu này nhằm mục đích tuyển chọn được các chủng nấm nội sinh trên cây Thạch tùng javanica Việt Nam có khả năng sinh đa enzym ngoại bào và đa kháng các VSV gây bệnh bằng phương pháp xác định khả năng sinh enzym ngoại bào của các chủng vi nấm và khuếch tán trên đĩa thạch. Kết quả cho thấy, 9 chủng nghiên cứu đều có khả năng sinh từ 1 - 5 loại enzym và ức chế từ 1 - 5 loài VSV gây bệnh với hoạt tính tiềm năng
2 chủng TLC11 và TLC9 sinh 4 ÷ 5 enzym (cellulase, lipase, protease, phosphatase, β-galactosidase) với đường kính vòng thủy phân cao nhất 22 ÷ 25 mm (protease) và 20 ÷ 23 mm (lipase)
chủng TLC13 ức chế cả 5 chủng VSV kiểm định (Escherichia coli, Staphylococcus aureus, Candida albican, Bacillus cereus, Pseudomonas aerigunosa) với hoạt tính cao nhất với 3 loài B. cereus (24 ± 1,2 mm), P. aerigunosa (26 ± 1,1 mm) và C. albican (36 ± 1,5 mm)
2 chủng TLC10 và TLC19 kháng 4/5 VSV kiểm định ngoại trừ S. aureus (TLC10) và E. coli (TLC19). Các chủng này sẽ là nguồn nguyên liệu tiềm năng trong các nghiên cứu sâu hơn nhằm hướng tới có thể ứng dụng xa hơn trong các lĩnh vực sản xuất nông-công-ngư nghiệp bền vững.

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