NGHIÊN CỨU CHIẾT TÁCH VÀ PHÂN GIẢI ELLAGITANNIN THU NHẬN AXIT ELLAGIC TỪ VỎ QUẢ LỰU TRẮNG

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Tác giả: Thanh Đức Hoàng, Thị Ngọc Hoàng

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học & Công nghệ - Trường Đại học Công nghiệp Hà Nội, 2024

Mô tả vật lý: tr.79

Bộ sưu tập: Báo, Tạp chí

ID: 389221

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Giống lựu trắng có lớp vỏ dày được trồng phổ biến tại nhiều nơi ở Việt Nam. Vỏ quả lựu có chứa nhiều hợp chất phenolic và ellagitannin. Ellagitannin là nhóm hợp chất tanin có thể thủy phân được để tạo ra axit ellagic tự nhiên. Trong nghiên cứu này, ellagitannin được tách chiết từ vỏ quả lựu trắng ở huyện Hoài Đức, thành phố Hà Nội, bằng dung môi ethanol 60%, ở nhiệt độ 80oC trong 3 giờ. Bằng cách thủy phân ellagitannin trong dung dịch axit H2SO4 5%, ở nhiệt độ 105oC trong thời gian 5 giờ thu được axit ellagic. Axit ellagic được tinh chế lại bằng cách kết tinh lặp lại nhiều lần trong methanol, với tỷ lệ 30ml methanol/1,0g axit ellagic thô. Loại dung môi và nồng độ dung môi dùng để chiết ellagitannin, nồng độ dung dịch axit H2SO4 và thời gian thủy phân ellagitannin để thu được axit ellagic với hiệu suất cao đã được khảo sát lựa chọn. Cấu tạo của axit ellagic được kiểm tra bằng các dữ kiện phổ hồng ngoại và phổ cộng hưởng từ hạt nhân. Hàm lượng của axit ellagic được xác định bằng phương pháp phân tích sắc ký lỏng hiệu năng cao (HPLC). Kết quả nghiên cứu đã thu được 3,0 g axit ellagic hàm lượng 93,7% từ 100g bột vỏ quả lựu khô.White pomegranate variety with thick skin is popularly grown in many places in Vietnam. Pomegranate peel contains many phenolic compounds and ellagitannin. Ellagitannin are a group of tannin compounds that can be hydrolyzed to produce natural ellagic acid. In this study, ellagitannin were extracted from white pomegranate peels in Hoai Duc district, Hanoi city, using solvent ethanol 60%, at 80oC for 3 hours. By hydrolyzing ellagitannin in 5% H2SO4 solution, at 105oC for 5 hours, ellagic acid was obtained. Ellagic acid was purified by repeated crystallization in methanol, at a ratio of 30ml methanol/1.0g crude ellagic acid. The type of solvent and concentration of solvent used to extract ellagitannin, concentration of H2SO4 acid solution and hydrolysis time of ellagitannin to obtain ellagic acid with high yield were investigated and selected. The structure of ellagic acid was examined by infrared and nuclear magnetic resonance spectroscopy data. The content of ellagic acid was determined by high performance liquid chromatography (HPLC) analysis. Research results have obtained 3.0g of ellagic acid with 93.7% content from 100g of dried pomegranate peel powder.

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