NGHIÊN CỨU CHUYỂN HÓA SINH KHỐI LIGNOCELLULOSE TỪ MÙN GỖ SỬ DỤNG HỖN HỢP ENZYME THỦY PHÂN TỪ NẤM THU NHẬN CÁC HỢP CHẤT CARBOHYDRATE VÀ AXÍT HYDROXYCINNAMIC

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Tác giả: Đình Giáp Vũ

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học & Công nghệ - Trường Đại học Công nghiệp Hà Nội, 2024

Mô tả vật lý: tr.99

Bộ sưu tập: Báo, Tạp chí

ID: 390824

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Lignocellulose có vai trò quan trọng về mặt kinh tế khi được sử dụng như vật liệu thô cho các ứng dụng trong công nghệ sinh học và công nghiệp. Các loài nấm sợi được biết là có hệ enzyme thủy phân xúc tác hiệu quả giúp chúng phân hủy tốt lignocellulose. Trong nghiên cứu này, sử dụng hỗn hợp enzyme thủy phân tác dụng hiệp đồng để chuyển hóa mùn gỗ thành các sản phẩm có phân tử lượng nhỏ như axít hydroxycinnamic hay các hợp chất carbohydrate là những sản phẩm có giá trị được ứng dụng trong nhiều lĩnh vực. Các enzyme sử dụng gồm, Celluclast® 1.5L (hoạt tính riêng 700U/g), feruloyl esterase (AltFAE, 11,2U/mg) và acetyl esterase (XpoAE, 13,1U/mg). Quá trình chuyển hóa mùn gỗ giải phóng 0,55mg/g axit ferulic và 0,49mg/g p-coumaric (axit hydroxycinnamic) và 14,97mg/g carbohydrate (galactose, glucose và xylose) sử dụng hỗn hợp enzyme (Celluclast® 1.5L
5U/gds, AltFAE
10 U/gds và XpoAE
10U/gds) sau khi ủ 8 giờ ở 40oC. Đồng thời, chứng minh được hoạt tính chống oxi hóa của axit hydroxycinnamic trên bản mỏng khi thử trên hệ thạch rắn-DPPH.Lignocellulose is economically important when used as a raw material for applications in biotechnology and industry. Fungi are known to possess an efficient catalytic hydrolytic enzyme system that helps them to effectively decompose lignocellulose. In this study, a hydrolytic enzyme mixture was used to convert sawdust into products that included low molecular weight compounds such as hydroxycinnamic acids and carbohydrate compounds, which are valuable and applicable in various fields such as pharmaceuticals and the food industry. The enzymes used included Celluclast® 1.5L (specific activity of 700U/g) purified from Trichoderma reesei, feruloyl esterase from Alternaria tenuissima (AltFAE, 11.2U/mg), and acetyl esterase from Xylaria polymorpha (XpoAE, 13.1U/mg). The process of converting sawdust released 0.55mg/g ferulic acid and 0.49mg/g p-coumaric acid, as well as 14.97mg/g total carbohydrates (galactose, glucose, and xylose) using the enzymes (Celluclast® 1.5L
5U/gds, AltFAE
10U/gds, and XpoAE
10U/gds) after 8 hours of incubation at 40°C. The study also demonstrated the antioxidant activity of the hydroxycinnamic acid compounds on thin-layer chromatography when tested in a solid-phase DPPH assay.

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