Xác định khả năng ức chế rotavirus của hoạt chất genipin

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Tác giả: Thanh Việt Nguyễn, Văn Thái Thân

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí khoa học và công nghệ - Đại học Nguyễn Tất Thành 2019

Mô tả vật lý: 28-33

Bộ sưu tập: Metadata

ID: 392193

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Tóm tắt
Chủ đề

Rotaviruses (RVs) là nguyên nhân chủ yếu gây bệnh tiêu chảy cấp tính đối với trẻ em trên toàn thế giới nhưng lại không có thuốc điều trị đặc hiệu. Genipin là hợp chất tự nhiên trong cây dành dành có nhiều đặc tính dược lí như khả năng kháng viêm, kháng apoptotic, kháng vi sinh vật và khối u. Trong nghiên cứu này, hoạt chất genipin được thử nghiệm hoạt tính ức chế RVs trong điều kiện in-vitro. Ngưỡng gây độc tế bào MA104 của genipin l ≥150μM/ml
Hiệu quả ức chế RVs của genipin sử dụng ở nồng độ 10-150μM/ml so với đối chứng lần lượt như sau: xử lí RVs với genipin trước v trước/sau gây nhiễm là 28.7-54.1% và 47.3-90.0%
xử lí tế bào MA104 với genipin sau gây nhiễm là 0-66%
và xử lí tế bào MA104 với genipin trước v trước/sau gây nhiễm là 27.3-71.8% và 54.1-90%. Từ các kết quả trên cho thấy hoạt chất genipin có thể được sử dụng để ngăn ngừa và hạn chế sự xâm nhiễm RVs., Tóm tắt tiếng anh, Rotavirus is the major cause of acute gastroenteritis in children worldwide. So far, there has been no specific medicine of the treatment of this virus. Genipin is a chemical compound found in the natural fruit of gardenia and has been reported to be an excellent traditional oriental medicine that produces various pharmacological functions such as antiinflammatory, anti-apoptotic, anti-microbial, and anti-tumor effects. In this study, genipin compound was tested as a natural drug in activities of anti-rotavirus in in-vitro model. The potential cytotoxicity of the genipin against the MA104 cells was evaluated at ≥150μM/ml. Genipin titer used in this study ranged from 10 to 150μM/ml. Anti-rotavirus effects of genipin compared to controls are as follow: pre-treated RVs before and before/after infection reduced RVs infectivity by 28.7-54.1% and 47.3-90.0%
treated MA104 cells after infection reduced RVs infectivity by 0-66%
pre-treated MA104 cells before and before/after infection reduced RVs infectivity by 27.3-71.8% and 54.1-90%. These results suggested that genipin can be used as a natural and safe drug to inhibit rotavirus infection.

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