A research on cryopreservation of Phu Quoc dog semen

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Tác giả: Tuấn Dũng Nguyễn, Văn Thanh Nguyễn, Thị Chi Trần, Hải Yến Vũ

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

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ID: 393216

Thêm vào giỏ

Nghiên cứu thử nghiệm đông lạnh nhanh tinh dịch chó Phú Quốc đã được thực hiện nhằm phục vụ việc bảo tồn và nhân giống giống chó này bằng kỹ thuật phối tinh nhân tạo. (i) Chất lượng tinh của 6 con chó đực giống Phú Quốc được đánh giá, tinh được khai thác 6 lần/đực giống, 5 ngày lấy tinh một lần. Kết quả nghiên cứu cho thấy thể tích tinh dịch phân đoạn giàu tinh trùng là 1,42±0,13 ml
hoạt lực tinh trùng đạt 0,86±0,05 điểm
nồng độ tinh trùng đạt 604,7±85,0 triệu tinh trùng/ml, tổng số tinh trùng tiến thẳng/lần khai thác đạt 496,9±38,9 triệu tinh trùng, tỷ lệ tinh trùng kỳ hình là 11,1±0,9%. (ii) Đông lạnh nhanh các mẫu tinh đã được thực hiện theo quy trình CERCA (Benechet, 2007) trong môi trường TCG (Tris-Citrate-Glucose) có bổ sung 20% lòng đỏ trứng gà và thử nghiệm bổ sung chất chống choáng lạnh là glycerol (với các mức hàm lượng thử nghiệm là 0
4
6 và 8% thể tích) hoặc ethylene glycol (với các mức hàm lượng thử nghiệm là 0
6
8 và 10% thể tích)
tinh được đóng trong cọng rạ 0,25 ml chứa 100 triệu tinh trùng/liều tinh
sau 2 ngày bảo quản trong nitơ lỏng, các mẫu tinh được rã đông ở 37oC trong 1 phút. Kết quả xác định chất lượng tinh cho thấy hoạt lực tinh trùng sau rã đông đạt cao nhất trong môi trường chứa 6% glycerol và 8% ethylene glycol, đạt tương ứng là 0,46±0,02 và 0,49±0,03 điểm
tỷ lệ tinh trùng kỳ hình tương ứng là 24,61±0,39% và 22,86±0,33%., Tóm tắt tiếng anh, Study on Cryopreservation for Phu Quoc dog semen was conducted in order to serve for the conservation and propagation of this breed by artificial insemination. (i) The quality of sperm of 6 Phu Quoc dogs was evaluated. The semens were harvested in 6 times per sire, the interval between each harvesting time was 5 days. The studied results showed that the volume of semen containing highest number of sperms was 1.42±0.13 ml, sperm activity reached 0.86±0.05 points, sperm concentration was 604.7 ± 85.0 million sperms per 1 ml semen, of which the total number of sperms moving directly per each harvesting time reached 496.9 ± 38.9 million spermatozoa and the sperm abnormality rate was 11.1 ± 0.9%. The semen samples were packed in straws with 0.25 ml containing a total of 100 million sperm per dose and conserved in liquid nitrogen according to the CERCA procedure (Benechet, 2007) in TCG (Tris-Citrate-Glucose) medium supplemented 20% of egg yolk, with cryo-protectants, such as: glycerol (at the test levels: 0, 4, 6 and 8% by volume) or ethylene glycol (at the test levels: 0, 6, 8 and 10% by volume). After 2 days of conservation in liquid nitrogen, the semen samples were thawed at 370C for 1 minute. The result of testing sperm quality showed that the activity of sperm after thawing reached the highest level in the medium containing 6% glycerol or 8% ethylene glycol, that was 0.46 ± 0.02 and 49 ± 0.03 points, respectively, and the sperm abnormality rate was 24.61 ± 0.39% and 22.86 ± 0.33%, respectively

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