Biểu hiện gen mã hóa Galactinol synthase của lúa trong cây ngô Zea may L.

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Tác giả: Thị Thu Huệ Huỳnh, Duy Phương Nguyễn, Thu Hằng Phạm, Xuân Hội Phạm

Ngôn ngữ: Vie

Ký hiệu phân loại: 576.5 Genetics

Thông tin xuất bản: Sinh học 2019

Mô tả vật lý: 101-109

Bộ sưu tập: Metadata

ID: 395488

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Phân lập gen GolS từ thư viện cDNA xử lý điều kiện hạn của giống lúa Mộc tuyền (đặt tên là OsGolS). Để phục vụ mục tiêu tạo giống cây trồng biến đổi gen chống chịu hạn, đã thiết kế cấu trúc vector biểu hiện OsGolS và chuyển vào cây ngô (Zea mays) mô hình thông qua vi khuẩn Agrobacterium tumefaciens. Sự có mặt và số bản sao của gen chọn lọc HPT và gen đích OsGolS trong hệ gen của một số dòng ngô tái sinh đã được xác định bằng PCR and qPCR. Phân tích bằng RT-PCR đã chứng minh gen chuyển OsGolS đã được biểu hiện (ở mức độ mRNA) trong cây chuyển gen., Tóm tắt tiếng anh, Galactinol synthase (GolS) is a keybiological catalyst for the synthesis of the raffinose oligosaccharides (RFOs) which play important rolesin abiotic stress adaptationof plants, especially drought tolerance. GolSgene has been isolated on a variety of plants in order to create material resources for generatingtransgenic plants resistant to adverse environmental factors. In our previous research, we have isolatedaGolSgenefromdrought stress cDNA libraryof Oryza sativa L.Moctuyen (named OsGolS). In this study, the expression vector pCAM-Rd/OsGolS carrying the isolated OsGolSgene under the control of stress-inducible Rd29A promoter was constructed and introduced into Agrobacterium tumefaciensLBA4404, which wasused for maize transformation.PCR and Real-time PCR assay indicated that transgene wasintegrated in the genome of the regeneratedZea maysplants.Reverse transcription-PCR showed that the OsGolSwas transcribed into mRNA in Zea maysand was highly expressed. These results provide a basis for the study of the function of OsGolSin drought responses and for the development of drought stress tolerant crops.

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