Nhân giống in vitro lan kim tuyến (Anoectochilus sp.)

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Tác giả: Thị Mai Trinh Đỗ, Trần Phước Huy Nguyễn

Ngôn ngữ: Vie

Ký hiệu phân loại: 580.589 Plants

Thông tin xuất bản: Khoa học (Đại học Cần Thơ) 2023

Mô tả vật lý: 76-83

Bộ sưu tập: Metadata

ID: 398318

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Chủ đề

Lan kim tuyến (Anoectochilus sp.) thuộc họ Orchidaceae là một trong những loại dược liệu quý của Việt Nam. Dịch chiết chứa các hợp chất liên quan đến chức năng tim mạch như các arachidonic acid, β - sitosterol... Mục đích của nghiên cứu là thiết lập một quy trình nhân giống in vitro để bảo tồn và phát triển loài lan kim tuyến bằng cách sử dụng các chất điều hoà sinh trưởng (6-benzylaminopurine, thidiazudron) kết hợp với kỹ thuật cắt lát mỏng tế bào. Kết quả cho thấy môi trường Murashige & Skoog bổ sung 0,5 mg/L 6-benzylaminopurine kết hợp 0,5 mg/L thidiazuron là tối ưu cho sự phát sinh chồi lan kim tuyến với 3,67 chồi/mẫu. Kỹ thuật cắt lát mỏng tế bào theo chiều ngang kết hợp chiếu sáng ở 2.000 lux cho số lượng chồi phát sinh từ lát cắt cao nhất với 13,16 chồi/mẫu. Các chồi in vitro lan kim tuyến ra rễ tối ưu trên môi trường Murashige & Skoog giảm 25% cả đa lượng và vi lượng. Các kết quả này có thể sử dụng để nhân giống quy mô lớn cây lan kim tuyến., Tóm tắt tiếng anh, Anoectochilus sp., the Orchidaceae family, is one of the precious medicinal plants of Vietnam. Their extract contains several compounds related to cardiovascular functions such as arachidonic acid metabolites, β.- sitosterol, etc. The purpose of the study is to establish an in vitro propagation procedure for the conservation and development of Anoectochilus sp. using the plant growth regulators (6- benzylaminopurine, thidiazudron) combined with the thin cell layer technique have rapidly increased the number of shoots and rooting. The results showed that Murashige & Skoog medium supplemented with 0.5 mg/L 6-benzylaminopurine combined with 0.5 mg/L thidiazudron was optimal for the growth of Anoectochilus sp. shoots with 3.67 shoots/sample. The technique of transverse cell slicing combined with light intensity at 2,000 lux resulted in the highest number of shoots arising from the slice with 13.16 shoots/sample. In vitro shoots of Anoectochilus sp. with optimal rooting on Murashige & Skoog medium reduced by 25% in both macro and microelements. This system could be used for large- scale propagation of Anoectochillus sp.

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