Nghiên cứu nhân nhanh in vitro cây hoa hồng đổi màu (Rosa asagumo)

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Tác giả: Thị Thu Hương Bùi, Huy Giới Đồng

Ngôn ngữ: Vie

Ký hiệu phân loại: 580.589 Plants

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Lâm nghiệp, 2023

Mô tả vật lý: 28-35

Bộ sưu tập: Metadata

ID: 399510

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Tóm tắt
Chủ đề

Nghiên cứu này đã tiến hành đánh giá khả năng tạo mẫu sạch in vitro cây hoa hồng đổi màu (Rosa asagumo) và đánh giá ảnh hưởng của các chất điều tiết sinh trưởng như BAP, Kinetin, α-NAA đến khả năng nhân nhanh in vitro cây hoa hồng đổi màu. Kết quả thu được cho thấy: (i) 80% các đoạn thân mang mắt ngủ sống sạch khi được khử trùng lần lượt với Javel 5% trong 10 phút, Johnson 2,5% trong 10 phút và HgCl20,1% trong 2 phút
(ii) Hơn 90% đoạn thân mang mắt ngủ hoa hồng đổi màu tái sinh chồi in vitro khi nuôi cấy trên môi trường MS bổ sung 1,5 mg/l BAP và 1,0 mg/l Kinetin với hệ số tái sinh là 2,71 lần
(iii) Môi trường nhân nhanh chồi hoa hồng đổi màu in vitro thích hợp nhất là môi trường MS bổ sung 1,5 mg/l BAP, 1,0 mg/l Kinetin với hệ số nhân chồi là 2,79 lần
(iv) Chồi in vitro hoa hồng đổi màu nuôi trên môi trường ¼ MS cho tỉ lệ ra rễ đạt 96,64%, số rễ trung bình đạt 3,1 rễ/chồi, rễ mập, chiều dài trung bình là 2,91 cm., Tóm tắt tiếng anh, The project aims to improve the quality of the changeable color rose plantlets by studying the effects of plant growth regulators such as BAP, Kinetin, and NAA on its tissue culture. The results showed that i) 80% of the stem segments survived and were clean when being disinfected with Javel 5% for 10 minutes, then Johnson 2.5% for 10 minutes, and HgCl2 0.1% for 2 minutes. ii) The most optimal solution for shoot regeneration of the rose stem segments was the MS medium added 1.5 mg. L-1 BAP and 0.5 mg. L-1 Kinetin which resulted in a regeneration rate of over 90% and a regeneration coefficient was 2.71
iii) the MS medium supplemented with 1.5 mg. L-1 BAP and 1.0 mg. L-1 Kinetin was the most suitable for multiplying the shoots with a coefficient of 2.79
iv) and the ideal medium for rooting of the shoots was ¼ MS which stimulated 96.64% of samples rooting with 3.1 roots per shoot and an average of 2.19 cm in length.

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