Ứng dụng chỉ thị phân tử trong chọn giống lúa chịu mặn (Oryza sativaL)

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Tác giả: Chí Bửu Bùi, Thị Lang Nguyễn, Trọng Phước Nguyễn

Ngôn ngữ: Vie

Ký hiệu phân loại: 633 Field and plantation crops

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn , 2021

Mô tả vật lý: 45360

Bộ sưu tập: Metadata

ID: 403802

Thêm vào giỏ Liên kết toàn văn

Áp dụng bản đồ GGT từ quần thể BC3F3 trên tổ họp lai OM 1490/ Pokkali, mang gen chịu mặn thông qua ứng dụng chỉ thị phân tử để cải tiến giống lúa chống chịu mặn. Trong quần thể OM 1490/Pokkali có sự đa hình vói hai chỉ thị RM3252-S1-1 trên nhiễm sắc thể số 1 và RM223 trên nhiễm sắc thể số 8. Kết quả này có được nhờ sử dụng 31 chỉ thị trên nhiễm sắc thể số 1 với khoảng cách di truyền l-97cM và 21 chỉ thị phân tử trên nhiễm sắc thể số 8, khoảng cách di truyền từ 0-56 cM. Các cá thể con lai được lựa chọn phải mang gen đồng hợp trội trên vùng nhiễm sắc thể với 4 dòng BC3F3-11, BC3F3-40, BC3F3-51 và BC3F3-52 CÓ 100% vùng gen trùng họp vói cá thể bố (Pokkali), mang gen mục tiêu mặn nhờ GGT trên nhiễm sắc thể số 1 và năm dòng BC3F3-16, BC3F3-18, BC3F3-34, BC3F3-48 và BC3F3-51 trên nhiêm sắc thể số 8. Các cá thể được chọn có mang gen mặn và ghi nhận có dòng BC3F3-40, BC3F3-52 và BC3F3-51 cho năng suất cao nhất có ý nghĩa so với đối chứng. Do đó, kết quả này là những tài liệu tham khảo quan trọng phục vụ cho việc chọn tạo giống lúa chịu mặn., Tóm tắt tiếng anh, Climate change has had a great impact on rice crops in the Mekong delta. This paper mainly apply GGT from popualtion from BC3F3 populations on om 1490/Pokkali carrying tolerance sality through molecular markers application to improve salinity rice varieties. In the OM 1490/Pokkali population. There is a polymorphic on with two indicators RM3252-S1-1 on chromosome 1 and RM223 on chromosome 8. This result is achieved by using 31 indicators on chromo some 1 with a genetic distance of l-97cM and 21 molecular indicators on chromosome. 8, the genetic distance from 0-56 cM. The selected individuals must carry a superior co-gene on a chromosome that has 4 individuals with 100% of the genes that overlap with (Pokklai), carrying a salinity target gene. Selected hybrid individuals must carry dominant contracted genes on the chromosome region with 4 lines of BC3F3-H, BC3F3-40, BC3F3-5I and BC3F3-52 with 100% of the gene pool sterilization with the father individual (Pokkali), carrying the salt target gene thanks to GGT on chromosome and five lines BC3F3-I6, BC3F3-I8, BC3F3-34, BC3F3-48 and BC3F3-5I on chromosome 8. Selected individuals that carry salty genes and recorded yields have: BC3F3-40, BC3F3-52 and line BC3F3-5I. Thus, it provides important references for the cultivation of new varieties of tolerance sality rice.

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