Ảnh hưởng của việc bổ sung vegf đến sự thành thục nhân của tế bào trứng heo

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Tác giả: Ngọc Tấn Nguyễn, Thị Ngọc Hân Nguyễn

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học kỹ thuật chăn nuôi 2021

Mô tả vật lý: 45328

Bộ sưu tập: Metadata

ID: 405200

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Tóm tắt
Chủ đề

Nghiên cứu nhằm đánh giá ảnh hưởng của yếu tố tăng trưởng nội mao mạch (VEGF: Vascular Endothelial Growth Factor) đến sự thành thục nhân tế bào trứng heo. Phức hợp tế bào trứng (cumulus oocyte complexes-COCs) có từ 2 lớp tế bào cumulus trở lên và đồng đều tế bào chất sẽ được nuôi cấy thành thục trong 44 giờ. Môi trường nuôi cấy là TCM-199 có bổ sung 10% dịch nang noãn + 0,8% BSA và 10 UI hCG/ml. Protein VEGF được bổ sung ở các nồng độ khác nhau (50, 100, 200 ng/ml) và không bổ sung VEGF (0 ng/ml) được xem là đối chứng. Kết quả cho thấy tỷ lệ thành thục nhân của tế bào trứng heo tăng dần khi tăng nồng độ VEGF 0-200 ng/ml. Tỷ lệ thành thục nhân cao nhất ở nhóm COC nuôi cấy có bổ sung 200 ng/ml và thấp nhất ở nhóm đối chứng (82,9 so với 58,6%, P<
0,01) hoặc 65,7 hay 74,3% lần lượt ở nhóm COC bổ sung 50 và 100 ng/ml. Kết quả cũng cho thấy bổ sung protein VEGF (200 ng/ml) theo các giai đoạn nuôi cấy có ảnh hưởng đến tỷ lệ thành thục nhân. Tỷ lệ thành thục nhân cao nhất ở nhóm bổ sung VEGF suốt 44 giờ nuôi cấy (85,8%), tiếp đến là nhóm bổ sung ở 22 giờ đầu (75,0%), sau đó là nhóm bổ sung ở 22 giờ cuối (66,7%) và thấp nhất ở nhóm đối chứng (59,2%). Việc bổ sung VEGF giúp cải thiện tỷ lệ thành thục nhân tế bào trứng heo trong điều kiện in vitro, cần tiếp tục nghiên cứu để làm rõ hơn vai trò của VEGF đến thành thục tế bào chất và phát triển phôi sau đó., Tóm tắt tiếng anh, In the present study, the effects of Vascular Endothelial Growth Factor (VEGF) protein supplementation in culture medium on porcine oocytes nuclear maturation in vitro was investigated. Cumulus oocyte complexes (COCs) were collected and cultured in TCM-199 medium containing 10% follicle fluid (FF), 0.8% BSA and 10UI (hCG/ml) and supplemented with different concentrations of VEGF (0, 50, 100, 200 ng/ml). The results showed that treated group COCs with VEGF protein as 200 ng/ml resulted in enhanced nuclear maturation rate (82.9%) as compared to control group (58.6%) or other treated groups with 50 ng/ml (65.7%) and 100 ng/ml (74.3%). Furthermore, we also found the stage-dependent effect for VEGF supplemented in culture medium on nuclear maturation. The maturation rate was highest (P<
0.01) in the treated group during 44h of culture (85.8%) as compared to none treated group (59.2%), treated group just for first 22h (75.0%) or the last 22h (66.7%). Taken together, we conclude that supplemented as 200 ng/ml of VEGF in the culture medium increases the nuclear maturation rate of porcine oocytes. However, in order to understand more insight into the cytoplasmic maturation requires more studies.

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