Ảnh hưởng của chitosan lên sự sinh trưởng của lan mokara nuôi cấy mô

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Tác giả: Kim Đỉnh Huỳnh, Ngọc Thiên Trang Nguyễn, Thị Minh Thu Phạm

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí khoa học - công nghệ thủy sản - Đại học Nha Trang 2020

Mô tả vật lý: 85-93

Bộ sưu tập: Metadata

ID: 406129

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Mokara là loài lan đẹp, đa dạng về màu sắc, ưa khí hậu nóng, dai sức và dễ trồng. Vì vậy, có những thời điểm mokara được trồng và nhân giống rộng rãi, đặc biệt bằng phương pháp nuôi cấy mô. Tuy nhiên, gần đây cây cấy mô mokara ít được phát triển vì tốc độ phát triển chậm kể cả trong giai đoạn nhân giống in vitro và chăm sóc in vivo. Trong nghiên cứu này, chitosan được thêm vào môi trường nhân giống nhằm cải thiện tốc độ sinh trưởng của chồi lan, từ đó giảm bớt thời gian nuôi cấy mô. Chitosan tách chiết từ vỏ tôm và mai mực với 3 khoảng phân tử lượng (Mw <
10, 30-50 và 80-100 kDa, kí hiệu Mw10, Mw30 và Mw80) và 3 độ deacetyl (72-75, 82-85 và 92-95%, kí hiệu D70, D80 và D90) đã được khảo sát với tiêu chí kích thích chồi lan tăng trưởng về chiều cao. Kết quả cho thấy chitosan tôm, Mw30, D80 sử dụng ở nồng độ 20 ppm là phù hợp cho kích thích tạo chồi và kéo dài chồi. Như vậy, nghiên cứu đã minh hoạ cho tác dụng kích thích sinh trưởng lên thực vật in vitro nói chung của chitosan và mở ra một hướng ứng dụng cho đối tượng lan mokara nói riêng., Tóm tắt tiếng anh, Mokara orchid is a tropical species and easy to plant due to its vitality and endurance. The flowers are colorful and long-lasting. Thus, mokara has been intensively planted and propagated by both traditional and tissue culture methods. However, despite the rapid multiplication, the micropropagated mokara shoot and plantlets grow very slowly. In this report, chitosans extracted from shrimp shell and squid pen with 3 molecular weight ranges (Mw <
10, 30-50 and 80-100 kDa, coded as Mw10, Mw30 and Mw80
respectively) and 3 deacetylation degrees (72-75, 82-85 and 92-95%, coded as D70, D80 and D90
respectively) were added to the tissue culture medium to examine the ability to promote the tissue growth. As the result, chitosan originated from shrimp shell with Mw30 and D80 was more suitable for the purpose of enhancing shoot development. Applying this type of chitosan with the concentration of 20 ppm was the best for shoot multiplication and elongation. Thus, chitosan could be used as an elicitor for mokara in vitro growth.

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